ABC transporter mis-splicing associated with resistance to Bt toxin Cry2Ab in laboratory- and field-selected pink bollworm

Lolita G. Mathew, Jeyakumar Ponnuraj, Bheemanna Mallappa, Lingutla R. Chowdary, Jianwei - Zhang, Wee Tek Tay, Thomas K. Walsh, Karl H.J. Gordon, David G. Heckel, Sharon Downes, Yves Carriere, Xianchun Li, Bruce E Tabashnik, Jeffrey A. Fabrick

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Evolution of pest resistance threatens the benefits of genetically engineered crops that produce Bacillus thuringiensis (Bt) insecticidal proteins. Strategies intended to delay pest resistance are most effective when implemented proactively. Accordingly, researchers have selected for and analyzed resistance to Bt toxins in many laboratory strains of pests before resistance evolves in the field, but the utility of this approach depends on the largely untested assumption that laboratory- and field-selected resistance to Bt toxins are similar. Here we compared the genetic basis of resistance to Bt toxin Cry2Ab, which is widely deployed in transgenic crops, between laboratory- and field-selected populations of the pink bollworm (Pectinophora gossypiella), a global pest of cotton. We discovered that resistance to Cry2Ab is associated with mutations disrupting the same ATP-binding cassette transporter gene (PgABCA2) in a laboratory-selected strain from Arizona, USA, and in field-selected populations from India. The most common mutation, loss of exon 6 caused by alternative splicing, occurred in resistant larvae from both locations. Together with previous data, the results imply that mutations in the same gene confer Bt resistance in laboratory- and field-selected strains and suggest that focusing on ABCA2 genes may help to accelerate progress in monitoring and managing resistance to Cry2Ab.

Original languageEnglish (US)
Article number13531
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Pectinophora gossypiella
ABC transporters
Bacillus thuringiensis
toxins
pest resistance
mutation
insecticidal proteins
genes
alternative splicing
crops
exons
cotton
researchers
pests
genetically modified organisms
India
monitoring
larvae

ASJC Scopus subject areas

  • General

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ABC transporter mis-splicing associated with resistance to Bt toxin Cry2Ab in laboratory- and field-selected pink bollworm. / Mathew, Lolita G.; Ponnuraj, Jeyakumar; Mallappa, Bheemanna; Chowdary, Lingutla R.; Zhang, Jianwei -; Tay, Wee Tek; Walsh, Thomas K.; Gordon, Karl H.J.; Heckel, David G.; Downes, Sharon; Carriere, Yves; Li, Xianchun; Tabashnik, Bruce E; Fabrick, Jeffrey A.

In: Scientific Reports, Vol. 8, No. 1, 13531, 01.12.2018.

Research output: Contribution to journalArticle

Mathew, LG, Ponnuraj, J, Mallappa, B, Chowdary, LR, Zhang, J, Tay, WT, Walsh, TK, Gordon, KHJ, Heckel, DG, Downes, S, Carriere, Y, Li, X, Tabashnik, BE & Fabrick, JA 2018, 'ABC transporter mis-splicing associated with resistance to Bt toxin Cry2Ab in laboratory- and field-selected pink bollworm', Scientific Reports, vol. 8, no. 1, 13531. https://doi.org/10.1038/s41598-018-31840-5
Mathew, Lolita G. ; Ponnuraj, Jeyakumar ; Mallappa, Bheemanna ; Chowdary, Lingutla R. ; Zhang, Jianwei - ; Tay, Wee Tek ; Walsh, Thomas K. ; Gordon, Karl H.J. ; Heckel, David G. ; Downes, Sharon ; Carriere, Yves ; Li, Xianchun ; Tabashnik, Bruce E ; Fabrick, Jeffrey A. / ABC transporter mis-splicing associated with resistance to Bt toxin Cry2Ab in laboratory- and field-selected pink bollworm. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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