Abnormal recruitment of periendothelial cells to lymphatic capillaries in digestive organs of angiopoietin-2-deficient mice

Hiroshi Shimoda, Michael J Bernas, Marlys H Witte, Nicholas W. Gale, George D. Yancopoulos, Seiji Kato

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The fine structure of lymphatic capillaries in the digestive organs of angiopoietin-2 (Ang2) knockout mice was studied by using both immunohistochemistry and electron microscopy. The genetic deletion of Ang2 yielded hypoplasia and disorganization of the lymphatic capillaries, with their shapes being irregular, and an aberrant recruitment of vascular periendothelial cells immunopositive for smooth muscle actin to the lymphatic capillaries. The abnormal lymphatic periendothelial cells were considered to be a type of pericyte for the lymphatic capillaries after the deletion of Ang2, because they were ultrastructurally characterized by abundant thin myofilaments in their cytoplasm and long cytoplasmic extensions similar to those shown by blood vascular pericytes. The genetic replacement of Ang2 with Ang1 rescued the defects, viz., the disorganization and disordered structure of the lymphatic capillaries. The present findings suggest that Ang2 serves the morphogenesis of lymphatic capillaries as an agonist for the receptor, Tie2, and that Ang1 can replace Ang2 in guiding lymphatic formation and development.

Original languageEnglish (US)
Pages (from-to)329-337
Number of pages9
JournalCell & Tissue Research
Volume328
Issue number2
DOIs
Publication statusPublished - May 2007

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Keywords

  • Angiopoietin-2
  • Digestive organ
  • Electron microscopy
  • Immunohistochemistry
  • Lymphatic capillary
  • Mouse

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

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