ABO blood type predicts the cytolocalization of anti-P-glycoprotein monoclonal antibody reactivity in human colon and ureter

Ronald S. Weinstein, Jerome R. Kuszak, Shriram M. Jakate, Miriam D. Lebovitz, Larry F. Kluskens, John S. Coon

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Classic multidrug resistance is mediated by a P-glycoprotein. Using monoclonal antibody C219 (MAb C219) in an immunohistochemical study, we found high levels of putative Golgi P-glycoprotein in normal columnar and transitional epithelium in subpopulations of patients with specific blood types. For example, Golgi staining was present in blood type A patients in 46% of normal colon samples (N = 21) and 88% of normal ureter samples (N = 17). In comparison, Golgi staining was present in blood group O patients in only 6% of normal colon samples (N = 34) and in 0% of normal ureter samples (N = 19). The association of MAb C219 Golgi staining with blood type A and lack of Golgi staining with blood type O was statistically significant in normal colon (P = .001) and normal ureter (P < .0001). Inappropriate hyperexpression of P-glycoprotein was frequently found in colon carcinomas. Additional evidence that Golgi MAb C219 reactivity represents P-glycoprotein is presented. This includes (1) immunostaining of Golgi with two anti-P-glycoprotein MAbs, C219 and JSB-1, and (2) experiments in which Mab C219 Golgi reactivity was blocked by preincubation of MAb C219 with a specific P-glycoprotein epitope-containing peptide. The high degree of association of Golgi P-glycoprotein with blood type A may suggest a role for P-glycoprotein in processing or trafficking of specific blood group antigens.

Original languageEnglish (US)
Pages (from-to)949-958
Number of pages10
JournalHuman pathology
Volume21
Issue number9
DOIs
StatePublished - Sep 1990
Externally publishedYes

Keywords

  • P-glycoprotein
  • blood group substances
  • blood type
  • cancer therapy
  • carbohydrate antigens
  • chemoresistance
  • colon carcinoma
  • multidrug resistance

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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