Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast

Alden K. Henderson, Josephine Lai, Stephen H. Buck, Yutaka Fujiwara, Gurinder Singh, Mark S. Yamamura, Shigetada Nakanishi, William R. Roeske, Henry I. Yamamura

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Abstract

Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.

Original languageEnglish (US)
Pages (from-to)PL7-PL12
JournalLife Sciences
Volume47
Issue number3
DOIs
StatePublished - 1990

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Henderson, A. K., Lai, J., Buck, S. H., Fujiwara, Y., Singh, G., Yamamura, M. S., Nakanishi, S., Roeske, W. R., & Yamamura, H. I. (1990). Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast. Life Sciences, 47(3), PL7-PL12. https://doi.org/10.1016/0024-3205(90)90329-P