Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast

Alden K. Henderson, Josephine Lai, Stephen H. Buck, Yutaka Fujiwara, Gurinder Singh, Mark S. Yamamura, Shigetada Nakanishi, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.

Original languageEnglish (US)
JournalLife Sciences
Volume47
Issue number3
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Phosphatidylinositols
Hydrolysis
Communication
Fibroblasts
Transfection
Stomach
Complementary DNA
Clone Cells
Cells
Ligands
Cell Line
phosphatidylinositol receptors
senktide
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmacology

Cite this

Henderson, A. K., Lai, J., Buck, S. H., Fujiwara, Y., Singh, G., Yamamura, M. S., ... Yamamura, H. I. (1990). Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast. Life Sciences, 47(3). https://doi.org/10.1016/0024-3205(90)90329-P

Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast. / Henderson, Alden K.; Lai, Josephine; Buck, Stephen H.; Fujiwara, Yutaka; Singh, Gurinder; Yamamura, Mark S.; Nakanishi, Shigetada; Roeske, William R.; Yamamura, Henry I.

In: Life Sciences, Vol. 47, No. 3, 1990.

Research output: Contribution to journalArticle

Henderson, Alden K. ; Lai, Josephine ; Buck, Stephen H. ; Fujiwara, Yutaka ; Singh, Gurinder ; Yamamura, Mark S. ; Nakanishi, Shigetada ; Roeske, William R. ; Yamamura, Henry I. / Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast. In: Life Sciences. 1990 ; Vol. 47, No. 3.
@article{21d47c309ac44d7a9ba423401bfbb736,
title = "Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast",
abstract = "Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.",
author = "Henderson, {Alden K.} and Josephine Lai and Buck, {Stephen H.} and Yutaka Fujiwara and Gurinder Singh and Yamamura, {Mark S.} and Shigetada Nakanishi and Roeske, {William R.} and Yamamura, {Henry I.}",
year = "1990",
doi = "10.1016/0024-3205(90)90329-P",
language = "English (US)",
volume = "47",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast

AU - Henderson, Alden K.

AU - Lai, Josephine

AU - Buck, Stephen H.

AU - Fujiwara, Yutaka

AU - Singh, Gurinder

AU - Yamamura, Mark S.

AU - Nakanishi, Shigetada

AU - Roeske, William R.

AU - Yamamura, Henry I.

PY - 1990

Y1 - 1990

N2 - Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.

AB - Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=18744436966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18744436966&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(90)90329-P

DO - 10.1016/0024-3205(90)90329-P

M3 - Article

AN - SCOPUS:18744436966

VL - 47

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 3

ER -