Acetylcholine receptor clustering in C2 muscle cells requires chondroitin sulfate

I. Mook-Jung, Herman Gordon

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Proteoglycans have been implicated in the clustering of acetylcholine receptors (AChRs) on cultured myotubes and at the neuromuscular junction. We report that the presence of chondroitin sulfate is associated with the ability of cultured myotubes to form spontaneous clusters of AChRs. Three experimental manipulations of wild type C2 cells in culture were found to affect both glycosaminoglycans (GAGs) and AChR clustering in concert. Chlorate was found to have dose-dependent negative effects both on GAG sulfation and on the frequency of AChR clusters. When extracellular calcium was raised from 1.8 to 6.8 mM in cultures of wild-type C2 myotubes, increases were observed both in the level of cell layer-associated chondroitin sulfate and in the frequency of AChR clusters. Culture of wild-type C2 myotubes in the presence of chondroitinase ABC eliminated cell layer-associated chondroitin sulfate while leaving heparan sulfate intact and simultaneously prevented the formation of AChR clusters. Treatment with either chlorate or chondroitinase inhibited AChR clustering only if begun prior to the spontaneous formation of clusters. We propose that chondroitin sulfate plays an essential role in the initiation of AChR clustering and in the early events of synapse formation on muscle.

Original languageEnglish (US)
Pages (from-to)482-492
Number of pages11
JournalJournal of Neurobiology
Volume28
Issue number4
DOIs
StatePublished - 1995

Fingerprint

Chondroitin Sulfates
Cholinergic Receptors
Muscle Cells
Cluster Analysis
Skeletal Muscle Fibers
Chlorates
Chondroitinases and Chondroitin Lyases
Chondroitin ABC Lyase
Heparitin Sulfate
Neuromuscular Junction
Proteoglycans
Glycosaminoglycans
Synapses
Cell Culture Techniques
Calcium
Muscles

Keywords

  • acetylcholine receptor
  • calcium
  • chondroitin sulfate
  • clustering
  • synaptogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Acetylcholine receptor clustering in C2 muscle cells requires chondroitin sulfate. / Mook-Jung, I.; Gordon, Herman.

In: Journal of Neurobiology, Vol. 28, No. 4, 1995, p. 482-492.

Research output: Contribution to journalArticle

@article{87f35aa8a30248cd9a948f0c08b834e4,
title = "Acetylcholine receptor clustering in C2 muscle cells requires chondroitin sulfate",
abstract = "Proteoglycans have been implicated in the clustering of acetylcholine receptors (AChRs) on cultured myotubes and at the neuromuscular junction. We report that the presence of chondroitin sulfate is associated with the ability of cultured myotubes to form spontaneous clusters of AChRs. Three experimental manipulations of wild type C2 cells in culture were found to affect both glycosaminoglycans (GAGs) and AChR clustering in concert. Chlorate was found to have dose-dependent negative effects both on GAG sulfation and on the frequency of AChR clusters. When extracellular calcium was raised from 1.8 to 6.8 mM in cultures of wild-type C2 myotubes, increases were observed both in the level of cell layer-associated chondroitin sulfate and in the frequency of AChR clusters. Culture of wild-type C2 myotubes in the presence of chondroitinase ABC eliminated cell layer-associated chondroitin sulfate while leaving heparan sulfate intact and simultaneously prevented the formation of AChR clusters. Treatment with either chlorate or chondroitinase inhibited AChR clustering only if begun prior to the spontaneous formation of clusters. We propose that chondroitin sulfate plays an essential role in the initiation of AChR clustering and in the early events of synapse formation on muscle.",
keywords = "acetylcholine receptor, calcium, chondroitin sulfate, clustering, synaptogenesis",
author = "I. Mook-Jung and Herman Gordon",
year = "1995",
doi = "10.1002/neu.480280408",
language = "English (US)",
volume = "28",
pages = "482--492",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Acetylcholine receptor clustering in C2 muscle cells requires chondroitin sulfate

AU - Mook-Jung, I.

AU - Gordon, Herman

PY - 1995

Y1 - 1995

N2 - Proteoglycans have been implicated in the clustering of acetylcholine receptors (AChRs) on cultured myotubes and at the neuromuscular junction. We report that the presence of chondroitin sulfate is associated with the ability of cultured myotubes to form spontaneous clusters of AChRs. Three experimental manipulations of wild type C2 cells in culture were found to affect both glycosaminoglycans (GAGs) and AChR clustering in concert. Chlorate was found to have dose-dependent negative effects both on GAG sulfation and on the frequency of AChR clusters. When extracellular calcium was raised from 1.8 to 6.8 mM in cultures of wild-type C2 myotubes, increases were observed both in the level of cell layer-associated chondroitin sulfate and in the frequency of AChR clusters. Culture of wild-type C2 myotubes in the presence of chondroitinase ABC eliminated cell layer-associated chondroitin sulfate while leaving heparan sulfate intact and simultaneously prevented the formation of AChR clusters. Treatment with either chlorate or chondroitinase inhibited AChR clustering only if begun prior to the spontaneous formation of clusters. We propose that chondroitin sulfate plays an essential role in the initiation of AChR clustering and in the early events of synapse formation on muscle.

AB - Proteoglycans have been implicated in the clustering of acetylcholine receptors (AChRs) on cultured myotubes and at the neuromuscular junction. We report that the presence of chondroitin sulfate is associated with the ability of cultured myotubes to form spontaneous clusters of AChRs. Three experimental manipulations of wild type C2 cells in culture were found to affect both glycosaminoglycans (GAGs) and AChR clustering in concert. Chlorate was found to have dose-dependent negative effects both on GAG sulfation and on the frequency of AChR clusters. When extracellular calcium was raised from 1.8 to 6.8 mM in cultures of wild-type C2 myotubes, increases were observed both in the level of cell layer-associated chondroitin sulfate and in the frequency of AChR clusters. Culture of wild-type C2 myotubes in the presence of chondroitinase ABC eliminated cell layer-associated chondroitin sulfate while leaving heparan sulfate intact and simultaneously prevented the formation of AChR clusters. Treatment with either chlorate or chondroitinase inhibited AChR clustering only if begun prior to the spontaneous formation of clusters. We propose that chondroitin sulfate plays an essential role in the initiation of AChR clustering and in the early events of synapse formation on muscle.

KW - acetylcholine receptor

KW - calcium

KW - chondroitin sulfate

KW - clustering

KW - synaptogenesis

UR - http://www.scopus.com/inward/record.url?scp=0028850501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028850501&partnerID=8YFLogxK

U2 - 10.1002/neu.480280408

DO - 10.1002/neu.480280408

M3 - Article

C2 - 8592108

AN - SCOPUS:0028850501

VL - 28

SP - 482

EP - 492

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 4

ER -