Actin isoform and α(1B)-adrenoceptor gene expression in aortic and coronary smooth muscle is influenced by cyclical stretch

M. S. Lundberg, D. N. Sadhu, V. E. Grumman, W. M. Chilian, Kenneth Ramos

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The occurrence of vascular domains with specific biological and pharmacological characteristics suggests that smooth muscle cells in different arteries may respond differentially to a wide range of environmental stimuli. To determine if some of these vessel-specific differences may be attributable to mechano-sensitive gene regulation, the influence of cyclical stretch on the expression of actin isoform and α(1B)-adrenoceptor genes was examined in aortic and coronary smooth muscle cells. Cells were seeded on an elastin substrate and subjected to maximal stretching (24% elongation) and relaxation cycles at a frequency of 120 cycles/min in a Flexercell strain unit for 72 h. Total RNA was extracted and hybridized to radiolabeled cDNA probes to assess gene expression. Stretch caused a greater reduction of actin isoform mRNA levels in aortic smooth muscle cells as compared to cells from the coronary artery. Steady-state mRNA levels of α(1B)-adrenoceptor were also decreased by cyclical stretch in both cell types but the magnitude of the response was greater in coronary smooth muscle cells. No changes in α(1B)-adrenoceptor or β/γ-actin steady-state mRNA levels were observed in H4IIE cells, a nonvascular, immortalized cell line. The relative gene expression of heat shock protein 70 was not influenced by the cyclic stretch regimen in any of these cell types. These results suggest that stretch may participate in the regulation of gene expression in vascular smooth muscle cells and that this response exhibits some degree of cell-specificity.

Original languageEnglish (US)
Pages (from-to)595-600
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume31
Issue number8
StatePublished - 1995
Externally publishedYes

Fingerprint

Gene expression
Adrenergic Receptors
Smooth Muscle
Muscle
Actins
Protein Isoforms
Smooth Muscle Myocytes
Cells
Gene Expression
RNA Isoforms
Messenger RNA
HSP70 Heat-Shock Proteins
Elastin
Gene Expression Regulation
Vascular Smooth Muscle
Genes
Stretching
Blood Vessels
Elongation
Coronary Vessels

Keywords

  • α-adrenergic receptor
  • actin
  • cyclical stretch
  • gene expression
  • smooth muscle cells

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

Cite this

Actin isoform and α(1B)-adrenoceptor gene expression in aortic and coronary smooth muscle is influenced by cyclical stretch. / Lundberg, M. S.; Sadhu, D. N.; Grumman, V. E.; Chilian, W. M.; Ramos, Kenneth.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 31, No. 8, 1995, p. 595-600.

Research output: Contribution to journalArticle

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