Actin isoform and α1B-adrenoceptor gene expression in aortic and coronary smooth muscle is influenced by cyclical stretch

Martha S. Lundberg, Devaki N. Sadhu, Vicky E. Grumman, William M. Chilian, Kenneth S. Ramos

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The occurrence of vascular domains with specific biological and pharmacological characteristics suggests that smooth muscle cells in different arteries may respond differentially to a wide range of environmental stimuli. To determine if some of these vessel-specific differences may be attributable to mechano-sensitive gene regulation, the influence of cyclical stretch on the expression of actin isoform and α1B-adrenoceptor genes was examined in aortic and coronary smooth muscle cells. Cells were seeded on an elastin substrate and subjected to maximal stretching (24% elongation) and relaxation cycles at a frequency of 120 cycles/min in a Flexercell strain unit for 72 h. Total RNA was extracted and hybridized to radiolabeled cDNA probes to assess gene expression. Stretch caused a greater reduction of actin isoform mRNA levels in aortic smooth muscle cells as compared to cells from the coronary artery. Steady-state mRNA levels of α1B -adrenoceptor were also decreased by cyclical stretch in both cell types but the magnitude of the response was greater in coronary smooth muscle cells. No changes in α1B-adrenoceptor or β/γ-actin steady-state mRNA levels were observed in H4IIE cells, a nonvascular, immortalized cell line. The relative gene expression of heat shock protein 70 was not influenced by the cyclic stretch regimen in any of these cell types. These results suggest that stretch may participate in the regulation of gene expression in vascular smooth muscle cells and that this response exhibits some degree of cell-specificity.

Original languageEnglish (US)
Pages (from-to)595-600
Number of pages6
JournalIn Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology
Volume31
Issue number8
DOIs
StatePublished - Sep 1995

Keywords

  • actin
  • cyclical stretch
  • gene expression
  • smooth muscle cells
  • α-adrenergic receptor

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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