ACTIN-RELATED PROTEIN6 regulates female meiosis by modulating meiotic gene expression in Arabidopsis

Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu

Research output: Research - peer-reviewArticle

  • 17 Citations

Abstract

In flowering plants, meiocytes develop from subepidermal cells in anthers and ovules. The mechanisms that integrate gene-regulatory processes with meiotic programs during reproductive development remain poorly characterized. Here, we show that Arabidopsis thaliana plants deficient in ACTIN-RELATED PROTEIN6 (ARP6), a subunit of the SWR1 ATP-dependent chromatin-remodeling complex, exhibit defects in prophase I of female meiosis. We found that this meiotic defect is likely due to dysregulated expression of meiotic genes, particularly those involved in meiotic recombination, including DMC1 (DISRUPTED MEIOTIC cDNA1). Analysis of DMC1 expression in arp6 mutant plants indicated that ARP6 inhibits expression of DMC1 in the megasporocyte and surrounding nonsporogeneous ovule cells before meiosis. After cells enter meiosis, however, ARP6 activates DMC1 expression specifically in the megasporocyte even as it continues to inhibit DMC1 expression in the nonsporogenous ovule cells. We further show that deposition of the histone variant H2A.Z, mediated by the SWR1 chromatin-remodeling complex at the DMC1 gene body, requires ARP6. Therefore, ARP6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development.

LanguageEnglish (US)
Pages1612-1628
Number of pages17
JournalPlant Cell
Volume26
Issue number4
DOIs
StatePublished - 2014

Fingerprint

meiosis
actin
Arabidopsis
gene expression
Meiosis
Actins
Gene Expression
ovules
cells
Ovule
chromatin
genes
Chromatin Assembly and Disassembly
prophase
regulator genes
histones
anthers
Angiospermae
Arabidopsis thaliana
mutants

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Qin, Y., Zhao, L., Skaggs, M. I., Andreuzza, S., Tsukamoto, T., Panoli, A., ... Palanivelu, R. (2014). ACTIN-RELATED PROTEIN6 regulates female meiosis by modulating meiotic gene expression in Arabidopsis. Plant Cell, 26(4), 1612-1628. DOI: 10.1105/tpc.113.120576

ACTIN-RELATED PROTEIN6 regulates female meiosis by modulating meiotic gene expression in Arabidopsis. / Qin, Yuan; Zhao, Lihua; Skaggs, Megan I.; Andreuzza, Sebastien; Tsukamoto, Tatsuya; Panoli, Aneesh; Wallace, Kirsten N.; Smith, Steven; Siddiqi, Imran; Yang, Zhenbiao; Yadegari, Ramin; Palanivelu, Ravishankar.

In: Plant Cell, Vol. 26, No. 4, 2014, p. 1612-1628.

Research output: Research - peer-reviewArticle

Qin, Y, Zhao, L, Skaggs, MI, Andreuzza, S, Tsukamoto, T, Panoli, A, Wallace, KN, Smith, S, Siddiqi, I, Yang, Z, Yadegari, R & Palanivelu, R 2014, 'ACTIN-RELATED PROTEIN6 regulates female meiosis by modulating meiotic gene expression in Arabidopsis' Plant Cell, vol 26, no. 4, pp. 1612-1628. DOI: 10.1105/tpc.113.120576
Qin Y, Zhao L, Skaggs MI, Andreuzza S, Tsukamoto T, Panoli A et al. ACTIN-RELATED PROTEIN6 regulates female meiosis by modulating meiotic gene expression in Arabidopsis. Plant Cell. 2014;26(4):1612-1628. Available from, DOI: 10.1105/tpc.113.120576
Qin, Yuan ; Zhao, Lihua ; Skaggs, Megan I. ; Andreuzza, Sebastien ; Tsukamoto, Tatsuya ; Panoli, Aneesh ; Wallace, Kirsten N. ; Smith, Steven ; Siddiqi, Imran ; Yang, Zhenbiao ; Yadegari, Ramin ; Palanivelu, Ravishankar. / ACTIN-RELATED PROTEIN6 regulates female meiosis by modulating meiotic gene expression in Arabidopsis. In: Plant Cell. 2014 ; Vol. 26, No. 4. pp. 1612-1628
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