Action of RecBCD enzyme on holliday structures made by RecA

Berndt Müller, Paul E Boehmer, Peter T. Emmerson, Stephen C. West

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In vitro, Escherichia coli RecA protein acts upon gapped and partially homologous linear duplex DNA to generate recombination products linked by Holliday junctions. When strand exchange reactions are supplemented with purified RecBCD enzyme, we observe the formation of products that resemble "patch" recombinants. The formation of "splice" recombinant products was not observed. The individual subunits, RecB, RecC, or RecD, had no effect on RecA protein-mediated strand exchange nor on the Holliday junctions formed in the reaction. Analysis of the way in which patch products arise indicates exonucleolytic digestion of the linear arms of the recombination intermediates (α-stuctures) by RecBCD enzyme. We find no evidence for specific resolution events at the site of the Holliday junction by RecBCD enzyme using these DNA substrates.

Original languageEnglish (US)
Pages (from-to)19028-19033
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number28
StatePublished - Oct 5 1991
Externally publishedYes

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Exodeoxyribonuclease V
Cruciform DNA
Rec A Recombinases
Genetic Recombination
Escherichia coli Proteins
DNA
Escherichia coli
Digestion
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Müller, B., Boehmer, P. E., Emmerson, P. T., & West, S. C. (1991). Action of RecBCD enzyme on holliday structures made by RecA. Journal of Biological Chemistry, 266(28), 19028-19033.

Action of RecBCD enzyme on holliday structures made by RecA. / Müller, Berndt; Boehmer, Paul E; Emmerson, Peter T.; West, Stephen C.

In: Journal of Biological Chemistry, Vol. 266, No. 28, 05.10.1991, p. 19028-19033.

Research output: Contribution to journalArticle

Müller, B, Boehmer, PE, Emmerson, PT & West, SC 1991, 'Action of RecBCD enzyme on holliday structures made by RecA', Journal of Biological Chemistry, vol. 266, no. 28, pp. 19028-19033.
Müller B, Boehmer PE, Emmerson PT, West SC. Action of RecBCD enzyme on holliday structures made by RecA. Journal of Biological Chemistry. 1991 Oct 5;266(28):19028-19033.
Müller, Berndt ; Boehmer, Paul E ; Emmerson, Peter T. ; West, Stephen C. / Action of RecBCD enzyme on holliday structures made by RecA. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 28. pp. 19028-19033.
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