Activation of latent transgenes in Arabidopsis using a hybrid transcription factor

Dave Guyer, Ann Tuttle, Sabrina Rouse, Sandra Volrath, Marie Johnson, Sharon Potter, Jörn Görlach, Stephen A Goff, Lyle Crossland, Eric Ward

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A hybrid transcription factor comprising a fusion of the DNA-binding domain of Saccharomyces cerevisiae GAL4 and the transcription activation domain of maize Cl was expressed in stably transformed Arabidopsis. Additional transgenic lines were created containing test genes controlled by a synthetic promoter consisting of concatemeric copies of the cis-acting site recognized by GAL4 (UAS (g)) fused to a minimal promoter. The GAL4/Cl effector line was crossed to two lines containing a synthetic promoter/GUS fusion. Both histochemical staining and GUS activity assays indicate strong activation of GUS expression was achieved only after crossing. The GAL4/C1 effector line was also crossed to 15 lines containing a synthetic promoter/antisense adenylosuccinate synthetase gene. Severely retarded growth, and in some cases lethality, was observed in 40% of the F1 lines. This system of activation by crossing is generally useful for activating expression of test transgenes.

Original languageEnglish (US)
Pages (from-to)633-639
Number of pages7
JournalGenetics
Volume149
Issue number2
StatePublished - Jun 1998
Externally publishedYes

Fingerprint

Transgenes
Arabidopsis
Adenylosuccinate Synthase
Transcription Factors
Transcriptional Activation
Genes
Zea mays
Saccharomyces cerevisiae
Staining and Labeling
DNA
Growth

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Guyer, D., Tuttle, A., Rouse, S., Volrath, S., Johnson, M., Potter, S., ... Ward, E. (1998). Activation of latent transgenes in Arabidopsis using a hybrid transcription factor. Genetics, 149(2), 633-639.

Activation of latent transgenes in Arabidopsis using a hybrid transcription factor. / Guyer, Dave; Tuttle, Ann; Rouse, Sabrina; Volrath, Sandra; Johnson, Marie; Potter, Sharon; Görlach, Jörn; Goff, Stephen A; Crossland, Lyle; Ward, Eric.

In: Genetics, Vol. 149, No. 2, 06.1998, p. 633-639.

Research output: Contribution to journalArticle

Guyer, D, Tuttle, A, Rouse, S, Volrath, S, Johnson, M, Potter, S, Görlach, J, Goff, SA, Crossland, L & Ward, E 1998, 'Activation of latent transgenes in Arabidopsis using a hybrid transcription factor', Genetics, vol. 149, no. 2, pp. 633-639.
Guyer D, Tuttle A, Rouse S, Volrath S, Johnson M, Potter S et al. Activation of latent transgenes in Arabidopsis using a hybrid transcription factor. Genetics. 1998 Jun;149(2):633-639.
Guyer, Dave ; Tuttle, Ann ; Rouse, Sabrina ; Volrath, Sandra ; Johnson, Marie ; Potter, Sharon ; Görlach, Jörn ; Goff, Stephen A ; Crossland, Lyle ; Ward, Eric. / Activation of latent transgenes in Arabidopsis using a hybrid transcription factor. In: Genetics. 1998 ; Vol. 149, No. 2. pp. 633-639.
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