Activation of mitogen-activated protein kinase by the human prostaglandin EP3A receptor

Thomas H. Burkey, John W. Regan

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Mitogen-activated protein (MAP) kinases are involved with cellular proliferation, and while the traditional activators of these kinases have been the growth factor receptors, recent data indicate that G-protein coupled receptors which inhibit adenylyl cyclase can activate MAP kinases as well. We have recently cloned an alternative splice variant of a human receptor for prostaglandin E2 (PGE2) which inhibits adenylyl cyclase and as been defined as the EP3A (Brit. J. Pharmacol. 112:377, 1994). In the present study the ability of this receptor to activate MAP kinase was examined. In crude lysates of COS-7 cells transfected with the human EP3A, 1 μM PGE2 stimicrolated MAP kinase activity ∼1.3-fold with an EC50 of ∼6 nM. Ion exchange chromatography followed by immicronoblot analysis showed that the stimicrolation of MAP kinase activity co-fractionated with immicronoreactive MAP-2 kinase (ERK1). This activation of MAP kinase activity by the EP3A receptor may explain the proliferative actions of PGE2 in some tissues.

Original languageEnglish (US)
Pages (from-to)152-158
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume211
Issue number1
DOIs
StatePublished - Jun 6 1995

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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