Activation of Skeletal Muscle Satellite Cells and the Role of Fibroblast Growth Factor Receptors

Sally E. Johnson, Ronald E Allen

Research output: Contribution to journalArticle

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Abstract

Specific, high-affinity binding of FGF2 was evaluated in cultured skeletal muscle satellite cells from young (3- to 4-week-old) and adult (9- to 12-month-old) rats prior to the first division in culture. Specific binding of FGF2 was detected on satellite cells from young rats at 18 h postplating, the earliest time examined, but specific binding was not detectable until 42 h on satellite cells from old rats. This correlates well with the delayed entry into the cell cycle exhibited by adult satellite cells and with the ability of satellite cells from rats of these ages to proliferate in response to FGF2. Patterns of tyrosine phosphorylation in whole cell extracts, following stimulation by FGF2, indicated specific FGF2 phosphorylation of proteins of 150/145, 90, 42, and 35 kDa in cells from both age groups. Several growth factors were evaluated for their ability to stimulate early entry of adult satellite cells into the cell cycle, and none of the following growth factors were able to activate proliferation of these cells: FGF2, IGF-1, IGF-2, PDGF-BB, TGF-β1, or TGF-β2. In addition, specific binding of FGF2 to 48-h cultures of adult satellite cells was not stimulated by FGF2, IGF-1, IGF-2, PDGF-BB, or TGF-β2, and specific binding was significantly decreased (P < 0.05) by FGF2 and TGF-β2. Specific binding was significantly lower in cells treated with PDGF-BB than in cells treated with either form of IGF but was greater than in cells treated with FGF2 or TGF-β2. The results of these experiments suggest that expression of functional FGF receptors on the surface of satellite cells may represent an important step in the activation of quiescent satellite cells.

Original languageEnglish (US)
Pages (from-to)449-453
Number of pages5
JournalExperimental Cell Research
Volume219
Issue number2
DOIs
StatePublished - Aug 1995

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Skeletal Muscle Satellite Cells
Fibroblast Growth Factor Receptors
Fibroblast Growth Factor 2
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins
Cell Cycle
Phosphorylation
Cell Extracts

ASJC Scopus subject areas

  • Cell Biology

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Activation of Skeletal Muscle Satellite Cells and the Role of Fibroblast Growth Factor Receptors. / Johnson, Sally E.; Allen, Ronald E.

In: Experimental Cell Research, Vol. 219, No. 2, 08.1995, p. 449-453.

Research output: Contribution to journalArticle

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