Acute changes in sputum IL-10 following underground exposure to diesel exhaust

Jefferey L Burgess, Joy E. Fleming, Emmanuel M. Mulenga, Arun Josyula, Tracy A. Hysong, Philip J. Joggerst, Margaret Kurzius-Spencer, Hugh B. Miller

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Introduction. Although exposure to diesel exhaust has been linked with adverse health effects, little is known about the acute effects of exposure in the underground workplace. Methods. Cross-shift spirometry and sputum induction were completed on twelve subjects associated with comminuted rock removal (mucking) operations in an underground copper mine using diesel powered and pneumatic equipment on separate days, and sputum collected on a baseline non-exposure day as well. Results. For diesel operations, elemental carbon exposure averaged 538 ± 512 μg/m3 during the 1-2 hour operations. Sputum interleukin-10 decreased with diesel exhaust using one ELISA assay (3.69 v. 2.32 pg/ml, p = 0.015), but increased when measured with a different ELISA kit (0.18 v. 0.59 pg/ml, p = 0.019), consistent with an overall decline in IL-10 protein concentration but an increase in the biologically active form. Sputum interleukin-6 decreased with exposure to diesel exhaust, although this change lost statistical significance when restricted to non-smokers. There were no significant changes in spirometry, interleukins 1β, 4, and 8, tumor necrosis factor alpha or 8-hydroxy-2′-deoxyguanosine. Conclusion. High levels of diesel exhaust can result in rapid changes in sputum IL-10, suggesting possible protein modification.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalClinical Toxicology
Volume45
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Vehicle Emissions
Sputum
Interleukin-10
Spirometry
Pneumatic equipment
Copper mines
Enzyme-Linked Immunosorbent Assay
Interleukin-1
Interleukin-6
Assays
Proteins
Interleukin-8
Carbon
Tumor Necrosis Factor-alpha
Workplace
Interleukin-4
Rocks
Health
Copper
Equipment and Supplies

Keywords

  • Diesel exhaust
  • Interleukin 10
  • Mining
  • Sputum

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Burgess, J. L., Fleming, J. E., Mulenga, E. M., Josyula, A., Hysong, T. A., Joggerst, P. J., ... Miller, H. B. (2007). Acute changes in sputum IL-10 following underground exposure to diesel exhaust. Clinical Toxicology, 45(3), 255-260. https://doi.org/10.1080/15563650601072142

Acute changes in sputum IL-10 following underground exposure to diesel exhaust. / Burgess, Jefferey L; Fleming, Joy E.; Mulenga, Emmanuel M.; Josyula, Arun; Hysong, Tracy A.; Joggerst, Philip J.; Kurzius-Spencer, Margaret; Miller, Hugh B.

In: Clinical Toxicology, Vol. 45, No. 3, 03.2007, p. 255-260.

Research output: Contribution to journalArticle

Burgess, JL, Fleming, JE, Mulenga, EM, Josyula, A, Hysong, TA, Joggerst, PJ, Kurzius-Spencer, M & Miller, HB 2007, 'Acute changes in sputum IL-10 following underground exposure to diesel exhaust', Clinical Toxicology, vol. 45, no. 3, pp. 255-260. https://doi.org/10.1080/15563650601072142
Burgess, Jefferey L ; Fleming, Joy E. ; Mulenga, Emmanuel M. ; Josyula, Arun ; Hysong, Tracy A. ; Joggerst, Philip J. ; Kurzius-Spencer, Margaret ; Miller, Hugh B. / Acute changes in sputum IL-10 following underground exposure to diesel exhaust. In: Clinical Toxicology. 2007 ; Vol. 45, No. 3. pp. 255-260.
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