Abstract
We studied lung clearance of aerosolized technetium-labeled diethylenetriamine pentaacetic acid (99mTcDTPA), plasma concentrations of 6-keto-PGFlα and thromboxane B2 and pulmonary edema as indices of lung injury in rabbits exposed to cigarette smoke (CSE). Forty-six rabbits were randomly assigned to 4 groups: control sham smoke exposure (SS, N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N - 10), CSE (N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N - 10), CSE (N - 9), and CSE ibuprofen-pretreated (CSE-I, N - 19). Ibuprofen (cyclooxygenase eicosanoid inhibitor) was administered as a single daily intramuscular injection (25 mg/kg)for 7 days before the experiment. Cigarette or sham smoke was delivered by syringe in a series of 5, 10, 20, and 30 tidal volume breaths with a 15-min counting period between each subset of breaths to determine 99mTcDTPA biological half-life (T1/2). In the ibuprofen pretreated group, CSE caused significant decreases in 99mTcDTPA T1/2 and dynamic lung compliance. Furthermore, these changes in lung function were accompanied by severe injury to type I alveolar cell epithelium, pulmonary edema, and frequently death of the rabbits. These findings suggest that inhibition of the cyclooxygenase pathway before CSE exacerbates lung injury in rabbits.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 113-126 |
| Number of pages | 14 |
| Journal | Experimental Lung Research |
| Volume | 13 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1987 |
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ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Molecular Biology
- Clinical Biochemistry
Cite this
Acute cigarette smoke exposure causes lung injury in rabbits treated with ibuprofen. / Witten, Mark L.; Lemen, Richard J.; Quan, Stuart F; Sobonya, Richard E; Magarelli, Judith L.; Bruck, Denise C.
In: Experimental Lung Research, Vol. 13, No. 2, 1987, p. 113-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acute cigarette smoke exposure causes lung injury in rabbits treated with ibuprofen
AU - Witten, Mark L.
AU - Lemen, Richard J.
AU - Quan, Stuart F
AU - Sobonya, Richard E
AU - Magarelli, Judith L.
AU - Bruck, Denise C.
PY - 1987
Y1 - 1987
N2 - We studied lung clearance of aerosolized technetium-labeled diethylenetriamine pentaacetic acid (99mTcDTPA), plasma concentrations of 6-keto-PGFlα and thromboxane B2 and pulmonary edema as indices of lung injury in rabbits exposed to cigarette smoke (CSE). Forty-six rabbits were randomly assigned to 4 groups: control sham smoke exposure (SS, N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N - 10), CSE (N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N - 10), CSE (N - 9), and CSE ibuprofen-pretreated (CSE-I, N - 19). Ibuprofen (cyclooxygenase eicosanoid inhibitor) was administered as a single daily intramuscular injection (25 mg/kg)for 7 days before the experiment. Cigarette or sham smoke was delivered by syringe in a series of 5, 10, 20, and 30 tidal volume breaths with a 15-min counting period between each subset of breaths to determine 99mTcDTPA biological half-life (T1/2). In the ibuprofen pretreated group, CSE caused significant decreases in 99mTcDTPA T1/2 and dynamic lung compliance. Furthermore, these changes in lung function were accompanied by severe injury to type I alveolar cell epithelium, pulmonary edema, and frequently death of the rabbits. These findings suggest that inhibition of the cyclooxygenase pathway before CSE exacerbates lung injury in rabbits.
AB - We studied lung clearance of aerosolized technetium-labeled diethylenetriamine pentaacetic acid (99mTcDTPA), plasma concentrations of 6-keto-PGFlα and thromboxane B2 and pulmonary edema as indices of lung injury in rabbits exposed to cigarette smoke (CSE). Forty-six rabbits were randomly assigned to 4 groups: control sham smoke exposure (SS, N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N - 10), CSE (N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N - 10), CSE (N - 9), and CSE ibuprofen-pretreated (CSE-I, N - 19). Ibuprofen (cyclooxygenase eicosanoid inhibitor) was administered as a single daily intramuscular injection (25 mg/kg)for 7 days before the experiment. Cigarette or sham smoke was delivered by syringe in a series of 5, 10, 20, and 30 tidal volume breaths with a 15-min counting period between each subset of breaths to determine 99mTcDTPA biological half-life (T1/2). In the ibuprofen pretreated group, CSE caused significant decreases in 99mTcDTPA T1/2 and dynamic lung compliance. Furthermore, these changes in lung function were accompanied by severe injury to type I alveolar cell epithelium, pulmonary edema, and frequently death of the rabbits. These findings suggest that inhibition of the cyclooxygenase pathway before CSE exacerbates lung injury in rabbits.
UR - http://www.scopus.com/inward/record.url?scp=0023076950&partnerID=8YFLogxK
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U2 - 10.3109/01902148709064313
DO - 10.3109/01902148709064313
M3 - Article
C2 - 3311720
AN - SCOPUS:0023076950
VL - 13
SP - 113
EP - 126
JO - Experimental Lung Research
JF - Experimental Lung Research
SN - 0190-2148
IS - 2
ER -