Acute cigarette smoke exposure increases alveolar permeability in rabbits

M. L. Witten, R. J. Lemen, Stuart F Quan, Richard E Sobonya, H. Roseberry, J. L. Stevenson, J. Clayton

Research output: Contribution to journalArticle

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Abstract

We measured lung clearance of aerosolized technetium-labeled diethylenetriamine pentaacetic acid (99mTcDTPA) as an index of alveolar epithelial permeability in rabbits exposed to cigarette smoke. Eighteen rabbits were randomly assigned to 3 equal-size groups: control, all smoke exposure (ASE), and limited smoke exposure (LSE). Cigarette or sham smoke was delivered by syringe in a series of 5, 10, 20, and 30 tidal volume breaths with a 20-min counting period between each subset of breaths to determine 99mTcDTPA biologic half-life (T 1/2 ). Mean T 1/2 minimum (i.e., the smallest T 1/2 observed) was significantly lower (p < 0.05) for ASE and LSE rabbits than by control rabbits. We observed a significant difference at 20 and 30 breath exposures between the control and ASE group mean values (% baseline) for T 1/2 , arterial blood pressure, and peak airway pressure. A combination of light and electron microscopy showed focal alveolar edema and hemorrhage in the ASE and LSE group but no alveolar-capillary membrane damage. In summary, acute cigarette smoke exposure increases alveolar permeability as measured by 99mTcDTPA clearance, but there was no detectable ultrastructural alteration of the alveolar-capillary membrane.

Original languageEnglish (US)
Pages (from-to)321-325
Number of pages5
JournalAmerican Review of Respiratory Disease
Volume132
Issue number2
StatePublished - 1985

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Smoke
Tobacco Products
Permeability
Rabbits
Technetium Tc 99m Pentetate
Membranes
Tidal Volume
Syringes
Half-Life
Edema
Electron Microscopy
Arterial Pressure
Hemorrhage
Light
Pressure
Lung
Control Groups

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Witten, M. L., Lemen, R. J., Quan, S. F., Sobonya, R. E., Roseberry, H., Stevenson, J. L., & Clayton, J. (1985). Acute cigarette smoke exposure increases alveolar permeability in rabbits. American Review of Respiratory Disease, 132(2), 321-325.

Acute cigarette smoke exposure increases alveolar permeability in rabbits. / Witten, M. L.; Lemen, R. J.; Quan, Stuart F; Sobonya, Richard E; Roseberry, H.; Stevenson, J. L.; Clayton, J.

In: American Review of Respiratory Disease, Vol. 132, No. 2, 1985, p. 321-325.

Research output: Contribution to journalArticle

Witten, ML, Lemen, RJ, Quan, SF, Sobonya, RE, Roseberry, H, Stevenson, JL & Clayton, J 1985, 'Acute cigarette smoke exposure increases alveolar permeability in rabbits', American Review of Respiratory Disease, vol. 132, no. 2, pp. 321-325.
Witten, M. L. ; Lemen, R. J. ; Quan, Stuart F ; Sobonya, Richard E ; Roseberry, H. ; Stevenson, J. L. ; Clayton, J. / Acute cigarette smoke exposure increases alveolar permeability in rabbits. In: American Review of Respiratory Disease. 1985 ; Vol. 132, No. 2. pp. 321-325.
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