Acute hyperglycemic exacerbation of lung ischemia-reperfusion injury is mediated by receptor for advanced glycation end-products signaling

Damien J. LaPar, Vanessa A. Hajzus, Yunge Zhao, Christine L. Lau, Brent A. French, Irving L. Kron, Ashish K. Sharma, Victor E. Laubach

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The effects of acute hyperglycemia on lung ischemia-reperfusion (IR) injury and the role of receptor for advanced glycation endproducts (RAGE) signaling in this process are unknown. The objective of this study was twofold: (1) evaluate the impact of acute hyperglycemia on lung IR injury; and (2) determine ifRAGEsignaling is a mechanism of hyperglycemia-enhanced IR injury. We hypothesized that acute hyperglycemia worsens lung IR injury through a RAGE signaling mechanism. C57BL/6 wild-type (WT) and RAGE knockout (RAGE -/-) mice underwent sham thoracotomy or lung IR (1-h left hilar occlusionand 2-h reperfusion). Acute hyperglycemia was established by dextrose injection 30 minutes before ischemia. Lung injury was assessed by measuring lung function, cytokine expression in bronchoalveolar lavage fluid, leukocyte infiltration, and microvascular permeability via Evans blue dye. Mean blood glucose levels doubled in hyperglycemic mice 30 minutes after dextrose injection. Compared with IR in normoglycemic mice, IR in hyperglycemic mice significantly enhanced lung dysfunction, cytokine expression (TNF-α, keratinocyte chemoattractant, IL-6, monocyte chemotactic protein-1, regulated upon activation, normal T cell expressed and secreted), leukocyte infiltration, and microvascular permeability. Lung injury and dysfunction after IR were attenuated in normoglycemic RAGE -/- mice, and hyperglycemia failed to exacerbate IR injury in RAGE -/- mice. Thus, this study demonstrates that acute hyperglycemia exacerbates lung IR injury, whereas RAGE deficiency attenuates IR injury and also prevents exacerbation of IR injury in an acute hyperglycemic setting. These results suggest that hyperglycemia- enhanced lung IR injury is mediated, at least in part, by RAGE signaling, and identifies RAGE as a potential, novel therapeutic target to prevent post-transplant lung IR injury.

Original languageEnglish (US)
Pages (from-to)299-305
Number of pages7
JournalAmerican journal of respiratory cell and molecular biology
Volume46
Issue number3
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Keywords

  • Hyperglycemia
  • Inflammation
  • Lung transplant
  • Receptor for advanced glycation end-products

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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