Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds

E. G. Semenyuk, Monica Schmidt, R. N. Beachy, T. Moravec, T. Woodford-Thomas

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFPKDEL reporter protein upon addition of the chemical ligand, methoxyfenozide. The chemical gene switch activated gene expression under in vitro conditions in somatic cotyledonary embryos and zygotic seed embryos cultured from transgenic soybean plants, as well as in seeds in planta under greenhouse conditions. The efficiency of induction of GFP expression under different growth conditions was strongly influenced by the developmental stage of the seed and availability of the inducer. The formation of ER-derived GFP-containing protein bodies in seed storage parenchyma cells was correlated with the level of induced expression.

Original languageEnglish (US)
Pages (from-to)987-999
Number of pages13
JournalTransgenic Research
Volume19
Issue number6
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Ecdysone
ecdysone
Transgenes
Soybeans
transgenes
Seeds
soybeans
seeds
methoxyfenozide
glycinin
gene expression
protein bodies
seed storage
Embryonic Structures
Spermatophytina
Plantae
somatic embryos
Switch Genes
embryo (plant)
Chemical Models

Keywords

  • Inducible expression
  • Methoxyfenozide
  • Seed-specific genes
  • Soybean

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Agronomy and Crop Science
  • Animal Science and Zoology

Cite this

Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds. / Semenyuk, E. G.; Schmidt, Monica; Beachy, R. N.; Moravec, T.; Woodford-Thomas, T.

In: Transgenic Research, Vol. 19, No. 6, 12.2010, p. 987-999.

Research output: Contribution to journalArticle

Semenyuk, E. G. ; Schmidt, Monica ; Beachy, R. N. ; Moravec, T. ; Woodford-Thomas, T. / Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds. In: Transgenic Research. 2010 ; Vol. 19, No. 6. pp. 987-999.
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