Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fcγ receptors I and II/III

Francisco A. Bonilla, Ross M. Fujita, Vadim I Pivniouk, Andrew C. Chan, Raif S. Geha

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kDa) adapter protein is expressed in T cells and myeloid cells, whereas its homologue BLNK (B cell linker protein) is expressed in B cells. SLP-76 and BLNK link immunoreceptor tyrosine-based activation motif-containing receptors to signaling molecules that include phospholipase C-γ, mitogen-activated protein kinases, and the GTPases Ras and Rho. SLP-76 plays a critical role in T cell receptor, FcεRI and gpVl collagen receptor signaling, and participates in signaling via FcγR and killer cell inhibitory receptors. BLNK plays a critical role in B cell receptor signaling. We show that murine bone marrow-derived macrophages express both SLP-76 and BLNK. Selective ligation of FcγRI and FcγRII/III resulted in tyrosine phosphorylation of both SLP-76 and BLNK. SLP-76(-/-) bone marrow-derived macrophages display FcγR-mediated tyrosine phosphorylation of Syk, phospholipase C-γ2, and extracellular signal regulated kinases 1 and 2, and normal FcγR-dependent phagocytosis. These data suggest that both SLP-76 and BLNK are coupled to FcγR signaling in murine macrophages.

Original languageEnglish (US)
Pages (from-to)1725-1730
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number4
DOIs
StatePublished - Feb 15 2000
Externally publishedYes

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Fc Receptors
Macrophages
Type C Phospholipases
Tyrosine
B-Lymphocytes
Immunoreceptor Tyrosine-Based Activation Motif
Phosphorylation
Collagen Receptors
KIR Receptors
ras Proteins
Proteins
Mitogen-Activated Protein Kinase 3
src Homology Domains
Mitogen-Activated Protein Kinase 1
Myeloid Cells
T-Cell Antigen Receptor
Mitogen-Activated Protein Kinases
Phagocytosis
Ligation
Leukocytes

Keywords

  • Monocytes
  • Phagocytosis

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fcγ receptors I and II/III. / Bonilla, Francisco A.; Fujita, Ross M.; Pivniouk, Vadim I; Chan, Andrew C.; Geha, Raif S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 4, 15.02.2000, p. 1725-1730.

Research output: Contribution to journalArticle

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AU - Chan, Andrew C.

AU - Geha, Raif S.

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