Adaptor protein mediates dynamic pump assembly for bacterial metal efflux

Ace George Santiago, Tai Yen Chen, Lauren A. Genova, Won Jung, Alayna M.George Thompson, Megan M. Mcevoy, Peng Chen

Research output: Research - peer-reviewArticle

Abstract

Multicomponent efflux complexes constitute a primary mechanism for Gram-negative bacteria to expel toxic molecules for survival. As these complexes traverse the periplasm and link inner and outer membranes, it remains unclear how they operate efficiently without compromising periplasmic plasticity. Combining single-molecule superresolution imaging and genetic engineering, we study in living Escherichia coli cells the tripartite efflux complex CusCBA of the resistance-nodulation-division family that is essential for bacterial resistance to drugs and toxic metals. We find that CusCBA complexes are dynamic structures and shift toward the assembled form in response to metal stress. Unexpectedly, the periplasmic adaptor protein CusB is a key metal-sensing element that drives the assembly of the efflux complex ahead of the transcription activation of the cus operon for defending against metals. This adaptor protein-mediated dynamic pump assembly allows the bacterial cell for efficient efflux upon cellular demand while still maintaining periplasmic plasticity; this could be broadly relevant to other multicomponent efflux systems.

LanguageEnglish (US)
Pages6694-6699
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number26
DOIs
StatePublished - Jun 27 2017
Externally publishedYes

Fingerprint

Metals
Pumps
Proteins
Poisons
Plasticity
Molecules
Genetic engineering
Transcription
Bacteria
Chemical activation
Membranes
Imaging techniques
Pharmaceutical Preparations

Keywords

  • Metal sensing
  • Multicomponent efflux complex
  • Periplasmic adaptor protein
  • Single-molecule tracking
  • Substrate-responsive dynamic assembly

ASJC Scopus subject areas

  • General

Cite this

Adaptor protein mediates dynamic pump assembly for bacterial metal efflux. / Santiago, Ace George; Chen, Tai Yen; Genova, Lauren A.; Jung, Won; Thompson, Alayna M.George; Mcevoy, Megan M.; Chen, Peng.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 26, 27.06.2017, p. 6694-6699.

Research output: Research - peer-reviewArticle

Santiago, Ace George ; Chen, Tai Yen ; Genova, Lauren A. ; Jung, Won ; Thompson, Alayna M.George ; Mcevoy, Megan M. ; Chen, Peng. / Adaptor protein mediates dynamic pump assembly for bacterial metal efflux. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 26. pp. 6694-6699
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