Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells

Felicia Goodrum, Thomas Shenk, David A. Ornelles

Research output: Contribution to journalArticle

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Abstract

The localization of the adenovirus type 5 34-kDa E4 and 55-kDa E1B proteins was determined in the absence of other adenovirus proteins. When expressed by transfection in human, monkey, hamster, rat, and mouse cell lines, the E1B protein was predominantly cytoplasmic and typically was excluded from the nucleus. When expressed by transfection, the E4 protein accumulated in the nucleus. Strikingly, when coexpressed by transfection in human, monkey, or baby hamster kidney cells, the E1B protein colocalized in the nucleus with the E4 protein. A complex of the E4 and E1B proteins was identified by coimmunoprecipitation in transfected HeLa cells. By contrast to the interaction observed in primate and baby hamster kidney cells, the E4 protein failed to direct the E1B protein to the nucleus in rat and mouse cell lines as well as CHO and V79 hamster cell lines. This failure of the E4 protein to direct the nuclear localization of the E1B protein in REF-52 rat cells was overcome by fusion with HeLa cells. Within 4 h of heterokaryon formation and with protein synthesis inhibited, a portion of the E4 protein present in the REF-52 nuclei migrated to the HeLa nuclei. Simultaneously, the previously cytoplasmic E1B protein colocalized with the E4 protein in both human and rat cell nuclei. These results suggest that a primate cell- specific factor mediates the functional interaction of the E1B and E4 proteins of adenovirus.

Original languageEnglish (US)
Pages (from-to)6323-6335
Number of pages13
JournalJournal of Virology
Volume70
Issue number9
StatePublished - Sep 1996
Externally publishedYes

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Adenoviridae
Nuclear Proteins
Primates
Proteins
proteins
cells
hamsters
Cricetinae
transfection
Transfection
rats
cell lines
kidney cells
Adenovirus E4 Proteins
HeLa Cells
Cell Line
Adenovirus E1B Proteins
Haplorhini
monkeys
heterokaryon

ASJC Scopus subject areas

  • Immunology

Cite this

Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. / Goodrum, Felicia; Shenk, Thomas; Ornelles, David A.

In: Journal of Virology, Vol. 70, No. 9, 09.1996, p. 6323-6335.

Research output: Contribution to journalArticle

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