Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brain

Jean Rossier, Edward D French, Claude Gros, Scott Minick, Roger Guillemin, Floyd E. Bloom

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

After boiling and extraction with 4M Guanidine HCl to inactive endogenous peptidases, the anterior and neurointermediate lobes of the rat pituitary were analysed by gel filtration and a beta-endorphin radioimmunoassay which detects proopiocortin, beta-lipotropin and beta-endorphin with equimolar sensitivity. In the anterior lobe, immunoreactive (ir) beta-endorphin was eluted in three peaks: 10% of the total immunoreactivity had an apparent Mr of proopiocortin, 57% had the Mr of beta-lipotropin and 27% had the Mr of beta-endorphin. In the neurointermediate lobe, all beta-endorphin immunoreactivity is eluted in one peak with an apparent Mr of synthetic beta-endorphin. Total (ir) Beta-endorphin levels in the anterior lobe were increased by adrenalectomy and decreased after long term administration of dexamethasone (0.2 mg/kg for 16 days) or by stress (1 hour of inescapable footshock). Levels in the neurointermediate lobe were unchanged after these treatments. Neither adrenalectomy or long term administration of dexamethasone at 0.2 mg/kg/day for 16 days had any effects on the levels of (ir) Met5-enkephalin and (ir) Leu5-enkephalin in the striatum, hypothalamus and neurointermediate lobe. After larger doses of dexamethasone (1 mg/kg/each 12 hrs for 7 days) (ir) enkephalin levels were increased in the striatum (150% of controls) but not in hypothalamus or neurointermediate lobe. These data further support independent avenues of post-synthetic peptide processing in the brain and pituitary lobes.

Original languageEnglish (US)
Pages (from-to)2105-2112
Number of pages8
JournalLife Sciences
Volume25
Issue number24-25
DOIs
StatePublished - 1979
Externally publishedYes

Fingerprint

Intermediate Pituitary Gland
Opioid Peptides
beta-Endorphin
Adrenalectomy
Dexamethasone
Rats
Brain
Enkephalins
beta-Lipotropin
Pro-Opiomelanocortin
Hypothalamus
Guanidine
Boiling liquids
Radioimmunoassay
Gel Chromatography
Peptide Hydrolases
Gels
Peptides
Processing

ASJC Scopus subject areas

  • Pharmacology

Cite this

Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brain. / Rossier, Jean; French, Edward D; Gros, Claude; Minick, Scott; Guillemin, Roger; Bloom, Floyd E.

In: Life Sciences, Vol. 25, No. 24-25, 1979, p. 2105-2112.

Research output: Contribution to journalArticle

Rossier, Jean ; French, Edward D ; Gros, Claude ; Minick, Scott ; Guillemin, Roger ; Bloom, Floyd E. / Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brain. In: Life Sciences. 1979 ; Vol. 25, No. 24-25. pp. 2105-2112.
@article{075c22cbef6a49e997d8832d0c1bc2a4,
title = "Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brain",
abstract = "After boiling and extraction with 4M Guanidine HCl to inactive endogenous peptidases, the anterior and neurointermediate lobes of the rat pituitary were analysed by gel filtration and a beta-endorphin radioimmunoassay which detects proopiocortin, beta-lipotropin and beta-endorphin with equimolar sensitivity. In the anterior lobe, immunoreactive (ir) beta-endorphin was eluted in three peaks: 10{\%} of the total immunoreactivity had an apparent Mr of proopiocortin, 57{\%} had the Mr of beta-lipotropin and 27{\%} had the Mr of beta-endorphin. In the neurointermediate lobe, all beta-endorphin immunoreactivity is eluted in one peak with an apparent Mr of synthetic beta-endorphin. Total (ir) Beta-endorphin levels in the anterior lobe were increased by adrenalectomy and decreased after long term administration of dexamethasone (0.2 mg/kg for 16 days) or by stress (1 hour of inescapable footshock). Levels in the neurointermediate lobe were unchanged after these treatments. Neither adrenalectomy or long term administration of dexamethasone at 0.2 mg/kg/day for 16 days had any effects on the levels of (ir) Met5-enkephalin and (ir) Leu5-enkephalin in the striatum, hypothalamus and neurointermediate lobe. After larger doses of dexamethasone (1 mg/kg/each 12 hrs for 7 days) (ir) enkephalin levels were increased in the striatum (150{\%} of controls) but not in hypothalamus or neurointermediate lobe. These data further support independent avenues of post-synthetic peptide processing in the brain and pituitary lobes.",
author = "Jean Rossier and French, {Edward D} and Claude Gros and Scott Minick and Roger Guillemin and Bloom, {Floyd E.}",
year = "1979",
doi = "10.1016/0024-3205(79)90204-2",
language = "English (US)",
volume = "25",
pages = "2105--2112",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "24-25",

}

TY - JOUR

T1 - Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brain

AU - Rossier, Jean

AU - French, Edward D

AU - Gros, Claude

AU - Minick, Scott

AU - Guillemin, Roger

AU - Bloom, Floyd E.

PY - 1979

Y1 - 1979

N2 - After boiling and extraction with 4M Guanidine HCl to inactive endogenous peptidases, the anterior and neurointermediate lobes of the rat pituitary were analysed by gel filtration and a beta-endorphin radioimmunoassay which detects proopiocortin, beta-lipotropin and beta-endorphin with equimolar sensitivity. In the anterior lobe, immunoreactive (ir) beta-endorphin was eluted in three peaks: 10% of the total immunoreactivity had an apparent Mr of proopiocortin, 57% had the Mr of beta-lipotropin and 27% had the Mr of beta-endorphin. In the neurointermediate lobe, all beta-endorphin immunoreactivity is eluted in one peak with an apparent Mr of synthetic beta-endorphin. Total (ir) Beta-endorphin levels in the anterior lobe were increased by adrenalectomy and decreased after long term administration of dexamethasone (0.2 mg/kg for 16 days) or by stress (1 hour of inescapable footshock). Levels in the neurointermediate lobe were unchanged after these treatments. Neither adrenalectomy or long term administration of dexamethasone at 0.2 mg/kg/day for 16 days had any effects on the levels of (ir) Met5-enkephalin and (ir) Leu5-enkephalin in the striatum, hypothalamus and neurointermediate lobe. After larger doses of dexamethasone (1 mg/kg/each 12 hrs for 7 days) (ir) enkephalin levels were increased in the striatum (150% of controls) but not in hypothalamus or neurointermediate lobe. These data further support independent avenues of post-synthetic peptide processing in the brain and pituitary lobes.

AB - After boiling and extraction with 4M Guanidine HCl to inactive endogenous peptidases, the anterior and neurointermediate lobes of the rat pituitary were analysed by gel filtration and a beta-endorphin radioimmunoassay which detects proopiocortin, beta-lipotropin and beta-endorphin with equimolar sensitivity. In the anterior lobe, immunoreactive (ir) beta-endorphin was eluted in three peaks: 10% of the total immunoreactivity had an apparent Mr of proopiocortin, 57% had the Mr of beta-lipotropin and 27% had the Mr of beta-endorphin. In the neurointermediate lobe, all beta-endorphin immunoreactivity is eluted in one peak with an apparent Mr of synthetic beta-endorphin. Total (ir) Beta-endorphin levels in the anterior lobe were increased by adrenalectomy and decreased after long term administration of dexamethasone (0.2 mg/kg for 16 days) or by stress (1 hour of inescapable footshock). Levels in the neurointermediate lobe were unchanged after these treatments. Neither adrenalectomy or long term administration of dexamethasone at 0.2 mg/kg/day for 16 days had any effects on the levels of (ir) Met5-enkephalin and (ir) Leu5-enkephalin in the striatum, hypothalamus and neurointermediate lobe. After larger doses of dexamethasone (1 mg/kg/each 12 hrs for 7 days) (ir) enkephalin levels were increased in the striatum (150% of controls) but not in hypothalamus or neurointermediate lobe. These data further support independent avenues of post-synthetic peptide processing in the brain and pituitary lobes.

UR - http://www.scopus.com/inward/record.url?scp=0018574003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018574003&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(79)90204-2

DO - 10.1016/0024-3205(79)90204-2

M3 - Article

C2 - 537470

AN - SCOPUS:0018574003

VL - 25

SP - 2105

EP - 2112

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 24-25

ER -