Adrenergic receptor stimulation suppresses oxidative metabolism in isolated rat islets and Min6 cells

Amy C. Kelly, Leticia E. Camacho, Ken Pendarvis, Hailey M. Davenport, Nathan R. Steffens, Kate E. Smith, Craig S. Weber, Ron Lynch, Klearchos K Papas, Sean W Limesand

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Insulin secretion is stimulated by glucose metabolism and inhibited by catecholamines through adrenergic receptor stimulation. We determined whether catecholamines suppress oxidative metabolism in β-cells through adrenergic receptors. In Min6 cells and isolated rat islets, epinephrine decreased oxygen consumption rates compared to vehicle control or co-administration of epinephrine with α2-adrenergic receptor antagonist yohimbine. Epinephrine also decreased forskolin-stimulated oxygen consumption rates, indicating cAMP dependent and independent actions. Furthermore, glucose oxidation rates were decreased with epinephrine, independent of the exocytosis of insulin, which was blocked with yohimbine. We evaluated metabolic targets through proteomic analysis after 4 h epinephrine exposure that revealed 466 differentially expressed proteins that were significantly enriched for processes including oxidative metabolism, protein turnover, exocytosis, and cell proliferation. These results demonstrate that acute α2-adrenergic stimulation suppresses glucose oxidation in β-cells independent of nutrient availability and insulin exocytosis, while cAMP concentrations are elevated. Proteomics and immunoblots revealed changes in electron transport chain proteins that were correlated with lower metabolic reducing equivalents, intracellular ATP concentrations, and altered mitochondrial membrane potential implicating a new role for adrenergic control of mitochondrial function and ultimately insulin secretion.

Original languageEnglish (US)
JournalMolecular and Cellular Endocrinology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Islets of Langerhans
Metabolism
Adrenergic Receptors
Epinephrine
Rats
Exocytosis
Insulin
Yohimbine
Glucose
Oxygen Consumption
Adrenergic Agents
Proteomics
Catecholamines
Metabolic Equivalent
Oxygen
Oxidation
Proteins
Adrenergic Antagonists
Mitochondrial Membrane Potential
Cell proliferation

Keywords

  • Adrenergic receptor
  • Insulin secretion
  • Oxidative phosphorylation
  • Pancreatic Islet
  • Proteomics
  • β-cell metabolism

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Adrenergic receptor stimulation suppresses oxidative metabolism in isolated rat islets and Min6 cells. / Kelly, Amy C.; Camacho, Leticia E.; Pendarvis, Ken; Davenport, Hailey M.; Steffens, Nathan R.; Smith, Kate E.; Weber, Craig S.; Lynch, Ron; Papas, Klearchos K; Limesand, Sean W.

In: Molecular and Cellular Endocrinology, 01.01.2018.

Research output: Contribution to journalArticle

Kelly, Amy C. ; Camacho, Leticia E. ; Pendarvis, Ken ; Davenport, Hailey M. ; Steffens, Nathan R. ; Smith, Kate E. ; Weber, Craig S. ; Lynch, Ron ; Papas, Klearchos K ; Limesand, Sean W. / Adrenergic receptor stimulation suppresses oxidative metabolism in isolated rat islets and Min6 cells. In: Molecular and Cellular Endocrinology. 2018.
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