Adsorption of a microtubule on a charged surface affects its disassembly dynamics

Yi Yang, Roberto Z Guzman, Pierre A Deymier, M. Umnov, J. Hoying, Srini Raghavan, O. Palusinski, B. J J Zelinski

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The dynamics of disassembly of microtubules deposited on surfaces is shown to be strongly dependent on the electrostatic interaction between the microtubule and the substrate. Fluorescence microscopy of microtubules adsorbed on a Poly-L-Lysine film and immersed in pure water show a drastic decrease in disassembly velocity compared to the microtubules in bulk water solutions. While microtubules suspended in pure water disassemble in seconds, the dissociation velocity of microtubules adsorbed on a Poly-L-Lysine film ranges from 0.8 to 1.0 μm/min in pure water. Kinetic Monte Carlo simulations of the microtubule dynamics indicate that a decrease in the dissociation velocity of unstable microtubules can be achieved by reducing the heterodimer dissociation rate constant of tubulin heterodimers constituting a single protofilament, adsorbed to the Poly-L-Lysine film. This model suggests that the reduction of the dissociation velocity originates from the electrostatic interactions between the positively charged amino groups of the Poly-L-Lysine film and the negatively charged microtubule surface.

Original languageEnglish (US)
Pages (from-to)2050-2056
Number of pages7
JournalJournal of Nanoscience and Nanotechnology
Volume5
Issue number12
DOIs
StatePublished - Dec 2005

Fingerprint

Microtubules
Adsorption
Lysine
Water
Coulomb interactions
Fluorescence microscopy
Tubulin
Static Electricity
Rate constants
Kinetics
Substrates
Fluorescence Microscopy

Keywords

  • Bottom-up
  • Charged surface
  • Disassembly
  • Interconnects
  • Microtubule dynamics
  • Monte carlo simulation
  • Poly-l-lysine

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Materials Science (miscellaneous)
  • Engineering (miscellaneous)

Cite this

Adsorption of a microtubule on a charged surface affects its disassembly dynamics. / Yang, Yi; Guzman, Roberto Z; Deymier, Pierre A; Umnov, M.; Hoying, J.; Raghavan, Srini; Palusinski, O.; Zelinski, B. J J.

In: Journal of Nanoscience and Nanotechnology, Vol. 5, No. 12, 12.2005, p. 2050-2056.

Research output: Contribution to journalArticle

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AU - Raghavan, Srini

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