Adsorption of DNA to mica, silylated mica, and minerals. Characterization by atomic force microscopy

M. Bezanilla, Srinivas Manne, D. E. Laney, Y. L. Lyubchenko, H. G. Hansma

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

The nature of DNA adsorption to various substrates has been investigated by atomic force microscopy. The substrate used were mica, silylated mica, calcite, fluorite, and barite. The presence of a divalent cation, either in solution or accessible to the DNA on the substrate, greatly improves DNA adsorption. A comparison of DNA adsorption from different buffers onto mica and silylated mica shows that buffer composition is much less important for DNA adsorption to silylated mica and also suggests that for good adsorption DNA must be electrostically adsorbed to the surface.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalLangmuir
Volume11
Issue number2
StatePublished - Feb 1995
Externally publishedYes

Fingerprint

Mica
mica
Minerals
Atomic force microscopy
DNA
deoxyribonucleic acid
minerals
atomic force microscopy
Adsorption
adsorption
Buffers
Substrates
buffers
Barium Sulfate
Barite
barite
Fluorspar
Calcium Carbonate
Calcite
Divalent Cations

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Bezanilla, M., Manne, S., Laney, D. E., Lyubchenko, Y. L., & Hansma, H. G. (1995). Adsorption of DNA to mica, silylated mica, and minerals. Characterization by atomic force microscopy. Langmuir, 11(2), 655-659.

Adsorption of DNA to mica, silylated mica, and minerals. Characterization by atomic force microscopy. / Bezanilla, M.; Manne, Srinivas; Laney, D. E.; Lyubchenko, Y. L.; Hansma, H. G.

In: Langmuir, Vol. 11, No. 2, 02.1995, p. 655-659.

Research output: Contribution to journalArticle

Bezanilla, M, Manne, S, Laney, DE, Lyubchenko, YL & Hansma, HG 1995, 'Adsorption of DNA to mica, silylated mica, and minerals. Characterization by atomic force microscopy', Langmuir, vol. 11, no. 2, pp. 655-659.
Bezanilla, M. ; Manne, Srinivas ; Laney, D. E. ; Lyubchenko, Y. L. ; Hansma, H. G. / Adsorption of DNA to mica, silylated mica, and minerals. Characterization by atomic force microscopy. In: Langmuir. 1995 ; Vol. 11, No. 2. pp. 655-659.
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