Adsorption of DNA to mica, silylated mica, and minerals. Characterization by atomic force microscopy

M. Bezanilla, Srinivas Manne, D. E. Laney, Y. L. Lyubchenko, H. G. Hansma

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Abstract

The nature of DNA adsorption to various substrates has been investigated by atomic force microscopy. The substrate used were mica, silylated mica, calcite, fluorite, and barite. The presence of a divalent cation, either in solution or accessible to the DNA on the substrate, greatly improves DNA adsorption. A comparison of DNA adsorption from different buffers onto mica and silylated mica shows that buffer composition is much less important for DNA adsorption to silylated mica and also suggests that for good adsorption DNA must be electrostically adsorbed to the surface.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalLangmuir
Volume11
Issue number2
Publication statusPublished - Feb 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Bezanilla, M., Manne, S., Laney, D. E., Lyubchenko, Y. L., & Hansma, H. G. (1995). Adsorption of DNA to mica, silylated mica, and minerals. Characterization by atomic force microscopy. Langmuir, 11(2), 655-659.