Adsorption of DNA to Mica, Silylated Mica, and Minerals: Characterization by Atomic Force Microscopy

Magdalena Bezanilla, Srinivas Manne, Daniel E. Laney, Yuri L. Lyubchenko, Helen G. Hansma

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

The nature of DNA adsorption to various substrates has been investigated by atomic force microscopy. The substrates used were mica, silylated mica, calcite, fluorite, and barite. The presence of a divalent cation, either in solution or accessible to the DNA on the substrate, greatly improves DNA adsorption. A comparison of DNA adsorption from different buffers onto mica and silylated mica shows that buffer compositionis much less important for DNA adsorption to silylated mica and also suggests that for good adsorption DNA must be electrostatically adsorbed to the surface.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalLangmuir
Volume11
Issue number2
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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