Abstract
Adsorption to goethite of extracellular polymeric substances from Bacillus subtilis Extracellular polymeric substances (EPS) are heterogeneous biopolymers produced by Gram-negative and Gram-positive bacterial cells. Adsorption of EPS to minerals can alter the substrata physico-chemistry and influence initial bacterial adhesion processes via conditioning film formation, but the effects of solution chemistry on uptake of EPS remain poorly understood. In this study, the adsorption to goethite (α-FeOOH) of EPS isolated from the early stationary growth-phase culture of Bacillus subtilis was investigated as a function of pH and ionic strength (I) in NaCl background electrolyte using batch studies coupled with Fourier transform infrared spectroscopy and size-exclusion high-performance liquid chromatography. Proteins, particularly those of higher molar mass, and phosphorylated macromolecules were adsorbed preferentially. Increasing solution I (1-100 mM NaCl) or pH (3.0-9.0) resulted in a decrease in the mass of EPS adsorbed. Batch studies and diffuse reflectance infrared Fourier transform spectra are consistent with ligand exchange of EPS phosphate groups for surface hydroxyls at Fe metal centers. The data indicate that both electrostatic and chemical bonding interactions contribute to selective fractionation of the EPS solution. Proteins and phosphate groups in phosphodiester bridges of nucleic acids likely play an important role in conditioning film formation at Fe oxide surfaces.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 827-838 |
| Number of pages | 12 |
| Journal | Geochimica et Cosmochimica Acta |
| Volume | 70 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 15 2006 |
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ASJC Scopus subject areas
- Geochemistry and Petrology
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Adsorption to goethite of extracellular polymeric substances from Bacillus subtilis. / Omoike, Anselm; Chorover, Jon.
In: Geochimica et Cosmochimica Acta, Vol. 70, No. 4, 15.02.2006, p. 827-838.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adsorption to goethite of extracellular polymeric substances from Bacillus subtilis
AU - Omoike, Anselm
AU - Chorover, Jon
PY - 2006/2/15
Y1 - 2006/2/15
N2 - Adsorption to goethite of extracellular polymeric substances from Bacillus subtilis Extracellular polymeric substances (EPS) are heterogeneous biopolymers produced by Gram-negative and Gram-positive bacterial cells. Adsorption of EPS to minerals can alter the substrata physico-chemistry and influence initial bacterial adhesion processes via conditioning film formation, but the effects of solution chemistry on uptake of EPS remain poorly understood. In this study, the adsorption to goethite (α-FeOOH) of EPS isolated from the early stationary growth-phase culture of Bacillus subtilis was investigated as a function of pH and ionic strength (I) in NaCl background electrolyte using batch studies coupled with Fourier transform infrared spectroscopy and size-exclusion high-performance liquid chromatography. Proteins, particularly those of higher molar mass, and phosphorylated macromolecules were adsorbed preferentially. Increasing solution I (1-100 mM NaCl) or pH (3.0-9.0) resulted in a decrease in the mass of EPS adsorbed. Batch studies and diffuse reflectance infrared Fourier transform spectra are consistent with ligand exchange of EPS phosphate groups for surface hydroxyls at Fe metal centers. The data indicate that both electrostatic and chemical bonding interactions contribute to selective fractionation of the EPS solution. Proteins and phosphate groups in phosphodiester bridges of nucleic acids likely play an important role in conditioning film formation at Fe oxide surfaces.
AB - Adsorption to goethite of extracellular polymeric substances from Bacillus subtilis Extracellular polymeric substances (EPS) are heterogeneous biopolymers produced by Gram-negative and Gram-positive bacterial cells. Adsorption of EPS to minerals can alter the substrata physico-chemistry and influence initial bacterial adhesion processes via conditioning film formation, but the effects of solution chemistry on uptake of EPS remain poorly understood. In this study, the adsorption to goethite (α-FeOOH) of EPS isolated from the early stationary growth-phase culture of Bacillus subtilis was investigated as a function of pH and ionic strength (I) in NaCl background electrolyte using batch studies coupled with Fourier transform infrared spectroscopy and size-exclusion high-performance liquid chromatography. Proteins, particularly those of higher molar mass, and phosphorylated macromolecules were adsorbed preferentially. Increasing solution I (1-100 mM NaCl) or pH (3.0-9.0) resulted in a decrease in the mass of EPS adsorbed. Batch studies and diffuse reflectance infrared Fourier transform spectra are consistent with ligand exchange of EPS phosphate groups for surface hydroxyls at Fe metal centers. The data indicate that both electrostatic and chemical bonding interactions contribute to selective fractionation of the EPS solution. Proteins and phosphate groups in phosphodiester bridges of nucleic acids likely play an important role in conditioning film formation at Fe oxide surfaces.
UR - http://www.scopus.com/inward/record.url?scp=31544451873&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31544451873&partnerID=8YFLogxK
U2 - 10.1016/j.gca.2005.10.012
DO - 10.1016/j.gca.2005.10.012
M3 - Article
AN - SCOPUS:31544451873
VL - 70
SP - 827
EP - 838
JO - Geochmica et Cosmochimica Acta
JF - Geochmica et Cosmochimica Acta
SN - 0016-7037
IS - 4
ER -