Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution

Vicki L. Sutherland; Jerilyn A. Timlin; Linda T. Nieman; John F. Guzowski; Monica K. Chawla; Paul F. Worley; Badri Roysam; Bruce L. McNaughton; Michael B. Sinclair; Carol A. Barnes

doi:10.1016/j.jneumeth.2006.08.018

Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution

Vicki L. Sutherland, Jerilyn A. Timlin, Linda T. Nieman, John F. Guzowski, Monica K. Chawla, Paul F. Worley, Badri Roysam, Bruce L. McNaughton, Michael B. Sinclair, Carol A. Barnes

Psychology

Research output: Contribution to journal › Article

22 Scopus citations

Abstract

Simultaneous imaging of multiple cellular components is of tremendous importance in the study of complex biological systems, but the inability to use probes with similar emission spectra and the time consuming nature of collecting images on a confocal microscope are prohibitive. Hyperspectral imaging technology, originally developed for remote sensing applications, has been adapted to measure multiple genes in complex biological tissues. A spectral imaging microscope was used to acquire overlapping fluorescence emissions from specific mRNAs in brain tissue by scanning the samples using a single fluorescence excitation wavelength. The underlying component spectra obtained from the samples are then separated into their respective spectral signatures using multivariate analyses, enabling the simultaneous quantitative measurement of multiple genes either at regional or cellular levels.

Original language	English (US)
Pages (from-to)	144-148
Number of pages	5
Journal	Journal of Neuroscience Methods
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/j.jneumeth.2006.08.018
State	Published - Feb 15 2007

Keywords

Fluorescence imaging
Hyperspectral imaging
Immediate early genes
Multivariate image analysis
Pushbroom line-imaging
Spectral unmixing

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/j.jneumeth.2006.08.018

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Sutherland, V. L., Timlin, J. A., Nieman, L. T., Guzowski, J. F., Chawla, M. K., Worley, P. F., Roysam, B., McNaughton, B. L., Sinclair, M. B., & Barnes, C. A. (2007). Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Journal of Neuroscience Methods, 160(1), 144-148. https://doi.org/10.1016/j.jneumeth.2006.08.018

Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. / Sutherland, Vicki L.; Timlin, Jerilyn A.; Nieman, Linda T.; Guzowski, John F.; Chawla, Monica K.; Worley, Paul F.; Roysam, Badri; McNaughton, Bruce L.; Sinclair, Michael B.; Barnes, Carol A.

In: Journal of Neuroscience Methods, Vol. 160, No. 1, 15.02.2007, p. 144-148.

Research output: Contribution to journal › Article

Sutherland, VL, Timlin, JA, Nieman, LT, Guzowski, JF, Chawla, MK, Worley, PF, Roysam, B, McNaughton, BL, Sinclair, MB & Barnes, CA 2007, 'Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution', Journal of Neuroscience Methods, vol. 160, no. 1, pp. 144-148. https://doi.org/10.1016/j.jneumeth.2006.08.018

Sutherland, Vicki L. ; Timlin, Jerilyn A. ; Nieman, Linda T. ; Guzowski, John F. ; Chawla, Monica K. ; Worley, Paul F. ; Roysam, Badri ; McNaughton, Bruce L. ; Sinclair, Michael B. ; Barnes, Carol A. / Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. In: Journal of Neuroscience Methods. 2007 ; Vol. 160, No. 1. pp. 144-148.

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