Adventitious shoot regeneration of scotch spearmint (Mentha x gracilis Sole)

Charleson R. Poovaiah, Stephen C. Weller, Matthew A. Jenks

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The effect of different cytokinins on in vitro adventitious shoot regeneration from internodal explants of Mentha X gracilis Sole (scotch spearmint) was investigated. Murashige and Skoog (MS) medium containing 100 mg l-1 myo-inositol, 0.4 mg l-1 thiamine-HCl, 2.0% (w/v) sucrose, 10% (v/v) coconut water and supplemented with 4.5 μM thidiazuron (TDZ) was effective in inducing adventitious shoot formation from callus. The greatest percentage of explants with shoots (85%) with the highest mean number of shoots per explant (29) was obtained with explants from the 1st and the 2nd internodes from 2-wk-old stock plants growing on a medium containing MS basal salts, 2% sucrose, 100 mg l-1 myo-inositol, 0.4 mg l-1 thiamine-HCl, at TDZ 4.5 μM and 10% (v/v) coconut water and solidified with 0.2% (w/v) phytagel. The regenerated shoots rooted on a medium containing MS basal salts, 100 mg l-1 myo-inositol, 0.4 mg l-1 thiamine-HCl, 2.0% sucrose, and 0.054 μM naphthalene acetic acid (NAA). Micropropagated plantlets were transplanted into soil and acclimated to greenhouse conditions. This is the first report describing adventitious shoot regeneration of scotch spearmint.

Original languageEnglish (US)
Pages (from-to)354-358
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume42
Issue number4
DOIs
StatePublished - Jul 2006
Externally publishedYes

Keywords

  • Cytokinins
  • Internodes
  • Micropropagation
  • Thidiazuron

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Fingerprint

Dive into the research topics of 'Adventitious shoot regeneration of scotch spearmint (Mentha x gracilis Sole)'. Together they form a unique fingerprint.

Cite this