Aflatoxin Binders I: In vitro binding assay for aflatoxin B1 by several potential sequestering agents

Duarte E. Diaz, Winston M. Hagler, Brinton A. Hopkins, Lon W. Whitlow

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Nine potential proprietary sequestering agents consisting of 4 activated charcoals, 3 sodium bentonites, a calcium bentonite, and an esterified glucomannan were compared in a novel in vitro assay for aflatoxin B1 (AFB1) binding. Agents were evaluated in 10% methanol prepared as 1% stirred suspensions at pH 3, 7, 10 and pH-unadjusted, with or without AFB1 at 5 μg/ml. All nine agents bound more than 95% of the 5 μg of AFB1 in solution, regardless of pH. The sodium bentonites bound 98, 95, and 98% of the AFB1. The four activated charcoals bound over 99%, the calcium bentonite bound 98%, and the esterified glucomannan bound 97% of the AFB1 in solution. The results suggested that the sequestering agents tested here had sufficient potential to bind AFB1 at pH values commonly found in the gastrointestinal tracts of ruminants and other animals.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalMycopathologia
Volume156
Issue number3
DOIs
StatePublished - May 15 2003
Externally publishedYes

Keywords

  • Activated charcoal
  • Aflatoxin B1
  • Calcium bentonite
  • Esterified glucomannan
  • Sodium bentonite

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)

Fingerprint Dive into the research topics of 'Aflatoxin Binders I: In vitro binding assay for aflatoxin B1 by several potential sequestering agents'. Together they form a unique fingerprint.

  • Cite this