Aflatoxin-free transgenic maize using host-induced gene silencing

Dhiraj Thakare, Jianwei - Zhang, Rod A Wing, Peter J. Cotty, Monica Schmidt

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Aflatoxins, toxic secondary metabolites produced by some Aspergillus species, are a universal agricultural economic problem and a critical health issue. Despite decades of control efforts, aflatoxin contamination is responsible for a global loss of millions of tons of crops each year. We show that host-induced gene silencing is an effective method for eliminating this toxin in transgenic maize. We transformed maize plants with a kernel-specific RNA interference (RNAi) gene cassette targeting the aflC gene, which encodes an enzyme in the Aspergillus aflatoxin biosynthetic pathway. After pathogen infection, aflatoxin could not be detected in kernels from these RNAi transgenic maize plants, while toxin loads reached thousands of parts per billion in nontransgenic control kernels. A comparison of transcripts in developing aflatoxin-free transgenic kernels with those from nontransgenic kernels showed no significant differences between these two groups. These results demonstrate that small interfering RNA molecules can be used to silence aflatoxin biosynthesis in maize, providing an attractive and precise engineering strategy that could also be extended to other crops to improve food security.

Original languageEnglish (US)
Article numbere1602382
JournalScience advances
Volume3
Issue number3
DOIs
StatePublished - Mar 1 2017

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Aflatoxins
Gene Silencing
Zea mays
Aspergillus
RNA Interference
Gene Targeting
Food Supply
Poisons
Genetically Modified Plants
Biosynthetic Pathways
Small Interfering RNA
Economics
Health
Enzymes
Infection
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Aflatoxin-free transgenic maize using host-induced gene silencing. / Thakare, Dhiraj; Zhang, Jianwei -; Wing, Rod A; Cotty, Peter J.; Schmidt, Monica.

In: Science advances, Vol. 3, No. 3, e1602382, 01.03.2017.

Research output: Contribution to journalArticle

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