Afterpulsing and its correction in fluorescence correlation spectroscopy experiments

Ming Zhao, Lei Jin, Bo Chen, Yao Ding, Hui Ma, Dieyan Chen

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Afterpulsing arises from feedback in a photon detector. This means that each real signal pulse can be followed by an afterpulse at a later time. This effect is particularly troubling in photon correlation experiments. Few treatments of this effect have appeared in the literature, and few software programs to solve the problem have been written. We demonstrate the afterpulsing effect in fluorescence correlation spectroscopy by using different avalanche photodiodes. We prove theoretically that under simple and reasonable conditions afterpulsing in autocorrelation can be eliminated to the leading order; we have found it easy to program software for the correction. We compare our results with those from cross correlation. We also discuss some experimental parameters that may affect the afterpulsing.

Original languageEnglish (US)
Pages (from-to)4031-4036
Number of pages6
JournalApplied Optics
Volume42
Issue number19
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Photons
Fluorescence
Spectroscopy
fluorescence
Avalanche photodiodes
Autocorrelation
spectroscopy
Experiments
Detectors
computer programs
Feedback
photons
avalanches
cross correlation
autocorrelation
photodiodes
detectors
pulses

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Zhao, M., Jin, L., Chen, B., Ding, Y., Ma, H., & Chen, D. (2003). Afterpulsing and its correction in fluorescence correlation spectroscopy experiments. Applied Optics, 42(19), 4031-4036.

Afterpulsing and its correction in fluorescence correlation spectroscopy experiments. / Zhao, Ming; Jin, Lei; Chen, Bo; Ding, Yao; Ma, Hui; Chen, Dieyan.

In: Applied Optics, Vol. 42, No. 19, 01.07.2003, p. 4031-4036.

Research output: Contribution to journalArticle

Zhao, M, Jin, L, Chen, B, Ding, Y, Ma, H & Chen, D 2003, 'Afterpulsing and its correction in fluorescence correlation spectroscopy experiments', Applied Optics, vol. 42, no. 19, pp. 4031-4036.
Zhao M, Jin L, Chen B, Ding Y, Ma H, Chen D. Afterpulsing and its correction in fluorescence correlation spectroscopy experiments. Applied Optics. 2003 Jul 1;42(19):4031-4036.
Zhao, Ming ; Jin, Lei ; Chen, Bo ; Ding, Yao ; Ma, Hui ; Chen, Dieyan. / Afterpulsing and its correction in fluorescence correlation spectroscopy experiments. In: Applied Optics. 2003 ; Vol. 42, No. 19. pp. 4031-4036.
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