Agarose isoelectrofocusing of intact virions

Katherine S. Zerda, Charles P Gerba

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A convenient and accurate method for determining the isoelectric points of intact virions is described. Tritium-labeled poliovirus 1 (strains Brunhilde and LSc-2) and echovirus 1 (isolates V239, V248, V212, R115 and 4CH-1) were successfully focused into sharp bands at their respective isoelectric points using a thin-layer agarose isoelectric focusing system. In situ detection of labeled virus bands in the agarose was by fluorography. Freezing and thawing of virus samples prior to isoelectric focusing did not alter their respective isoelectric points.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Virological Methods
Volume9
Issue number1
DOIs
StatePublished - 1984

Fingerprint

Isoelectric Point
Virion
Sepharose
Isoelectric Focusing
Photofluorography
Viruses
Human Enterovirus B
Poliovirus
Tritium
Freezing

Keywords

  • agarose IEF
  • isoelectric focusing
  • isoelectric points
  • virion pI

ASJC Scopus subject areas

  • Virology

Cite this

Agarose isoelectrofocusing of intact virions. / Zerda, Katherine S.; Gerba, Charles P.

In: Journal of Virological Methods, Vol. 9, No. 1, 1984, p. 1-6.

Research output: Contribution to journalArticle

@article{7d2cdb0d19834675b97ea010dac2a72b,
title = "Agarose isoelectrofocusing of intact virions",
abstract = "A convenient and accurate method for determining the isoelectric points of intact virions is described. Tritium-labeled poliovirus 1 (strains Brunhilde and LSc-2) and echovirus 1 (isolates V239, V248, V212, R115 and 4CH-1) were successfully focused into sharp bands at their respective isoelectric points using a thin-layer agarose isoelectric focusing system. In situ detection of labeled virus bands in the agarose was by fluorography. Freezing and thawing of virus samples prior to isoelectric focusing did not alter their respective isoelectric points.",
keywords = "agarose IEF, isoelectric focusing, isoelectric points, virion pI",
author = "Zerda, {Katherine S.} and Gerba, {Charles P}",
year = "1984",
doi = "10.1016/0166-0934(84)90077-6",
language = "English (US)",
volume = "9",
pages = "1--6",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Agarose isoelectrofocusing of intact virions

AU - Zerda, Katherine S.

AU - Gerba, Charles P

PY - 1984

Y1 - 1984

N2 - A convenient and accurate method for determining the isoelectric points of intact virions is described. Tritium-labeled poliovirus 1 (strains Brunhilde and LSc-2) and echovirus 1 (isolates V239, V248, V212, R115 and 4CH-1) were successfully focused into sharp bands at their respective isoelectric points using a thin-layer agarose isoelectric focusing system. In situ detection of labeled virus bands in the agarose was by fluorography. Freezing and thawing of virus samples prior to isoelectric focusing did not alter their respective isoelectric points.

AB - A convenient and accurate method for determining the isoelectric points of intact virions is described. Tritium-labeled poliovirus 1 (strains Brunhilde and LSc-2) and echovirus 1 (isolates V239, V248, V212, R115 and 4CH-1) were successfully focused into sharp bands at their respective isoelectric points using a thin-layer agarose isoelectric focusing system. In situ detection of labeled virus bands in the agarose was by fluorography. Freezing and thawing of virus samples prior to isoelectric focusing did not alter their respective isoelectric points.

KW - agarose IEF

KW - isoelectric focusing

KW - isoelectric points

KW - virion pI

UR - http://www.scopus.com/inward/record.url?scp=0021223582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021223582&partnerID=8YFLogxK

U2 - 10.1016/0166-0934(84)90077-6

DO - 10.1016/0166-0934(84)90077-6

M3 - Article

C2 - 6094606

AN - SCOPUS:0021223582

VL - 9

SP - 1

EP - 6

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 1

ER -