Agarose isoelectrofocusing of intact virions

Katherine S. Zerda, Charles P. Gerba

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

A convenient and accurate method for determining the isoelectric points of intact virions is described. Tritium-labeled poliovirus 1 (strains Brunhilde and LSc-2) and echovirus 1 (isolates V239, V248, V212, R115 and 4CH-1) were successfully focused into sharp bands at their respective isoelectric points using a thin-layer agarose isoelectric focusing system. In situ detection of labeled virus bands in the agarose was by fluorography. Freezing and thawing of virus samples prior to isoelectric focusing did not alter their respective isoelectric points.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Virological Methods
Volume9
Issue number1
DOIs
StatePublished - Aug 1984

Keywords

  • agarose IEF
  • isoelectric focusing
  • isoelectric points
  • virion pI

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Agarose isoelectrofocusing of intact virions'. Together they form a unique fingerprint.

  • Cite this