Albumin-like proteins are critical regulators of vascular redox signaling

Kenneth Ramos, Vilius Stribinskis, Marlene C. Steffen, Adrian Nanez, Diego Montoya-Durango, Qiang He

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This laboratory previously identified an albumin-like protein (denoted as p70) as a component of the macromolecular complex assembled within the 5′-regulatory region of redox-sensitive genes in vascular smooth muscle cells (vSMCs). Here we show that p70 is present in the cytosolic and nuclear compartments of vSMCs and dynamically responsive to redox status. Intense cytoplasmic and perinuclear staining, coupled with enhanced nuclear localization, was observed in vSMCs, but not HepG2 cells, treated with benzo(a)pyrene (BaP), H or N-acetylcysteine, agents known to modulate redox status. 3′ RACE indicated that p70 is not generated as a product of endogenous gene expression, but rather taken up from the extracellular compartment. While p70 was undetectable in cells grown for 24 hours under serum-free conditions, cell-associated, acid-resistant albumin was detected 30 min after the addition of exogenous albumin. vSMCs incubated at 4°C with 100 g/mL unlabeled BSA and 10 g/mL FITC-BSA for 60 minutes and switched to 37°C to examine temperature-sensitive label uptake showed punctate structures throughout the cell consistent with albumin internalization at the higher temperature. Albumin was found to influence redox-signaling, as evidenced by modulation of cyp1a1 gsta1 and Ha-ras gene inducibility. Together, these results implicate albumin and albumin-like proteins as critical regulators of vascular redox signaling.

Original languageEnglish (US)
Article number628615
JournalOxidative Medicine and Cellular Longevity
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Oxidation-Reduction
Blood Vessels
Albumins
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Proteins
Genes
Cells
Macromolecular Substances
Temperature
ras Genes
Nucleic Acid Regulatory Sequences
Benzo(a)pyrene
Hep G2 Cells
Acetylcysteine
Gene expression
Labels
Modulation
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology
  • Aging
  • Biochemistry

Cite this

Albumin-like proteins are critical regulators of vascular redox signaling. / Ramos, Kenneth; Stribinskis, Vilius; Steffen, Marlene C.; Nanez, Adrian; Montoya-Durango, Diego; He, Qiang.

In: Oxidative Medicine and Cellular Longevity, 2013.

Research output: Contribution to journalArticle

Ramos, Kenneth ; Stribinskis, Vilius ; Steffen, Marlene C. ; Nanez, Adrian ; Montoya-Durango, Diego ; He, Qiang. / Albumin-like proteins are critical regulators of vascular redox signaling. In: Oxidative Medicine and Cellular Longevity. 2013.
@article{546ffa7331b6455493a3e1c8271c75b7,
title = "Albumin-like proteins are critical regulators of vascular redox signaling",
abstract = "This laboratory previously identified an albumin-like protein (denoted as p70) as a component of the macromolecular complex assembled within the 5′-regulatory region of redox-sensitive genes in vascular smooth muscle cells (vSMCs). Here we show that p70 is present in the cytosolic and nuclear compartments of vSMCs and dynamically responsive to redox status. Intense cytoplasmic and perinuclear staining, coupled with enhanced nuclear localization, was observed in vSMCs, but not HepG2 cells, treated with benzo(a)pyrene (BaP), H or N-acetylcysteine, agents known to modulate redox status. 3′ RACE indicated that p70 is not generated as a product of endogenous gene expression, but rather taken up from the extracellular compartment. While p70 was undetectable in cells grown for 24 hours under serum-free conditions, cell-associated, acid-resistant albumin was detected 30 min after the addition of exogenous albumin. vSMCs incubated at 4°C with 100 g/mL unlabeled BSA and 10 g/mL FITC-BSA for 60 minutes and switched to 37°C to examine temperature-sensitive label uptake showed punctate structures throughout the cell consistent with albumin internalization at the higher temperature. Albumin was found to influence redox-signaling, as evidenced by modulation of cyp1a1 gsta1 and Ha-ras gene inducibility. Together, these results implicate albumin and albumin-like proteins as critical regulators of vascular redox signaling.",
author = "Kenneth Ramos and Vilius Stribinskis and Steffen, {Marlene C.} and Adrian Nanez and Diego Montoya-Durango and Qiang He",
year = "2013",
doi = "10.1155/2013/628615",
language = "English (US)",
journal = "Oxidative Medicine and Cellular Longevity",
issn = "1942-0900",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Albumin-like proteins are critical regulators of vascular redox signaling

AU - Ramos, Kenneth

AU - Stribinskis, Vilius

AU - Steffen, Marlene C.

AU - Nanez, Adrian

AU - Montoya-Durango, Diego

AU - He, Qiang

PY - 2013

Y1 - 2013

N2 - This laboratory previously identified an albumin-like protein (denoted as p70) as a component of the macromolecular complex assembled within the 5′-regulatory region of redox-sensitive genes in vascular smooth muscle cells (vSMCs). Here we show that p70 is present in the cytosolic and nuclear compartments of vSMCs and dynamically responsive to redox status. Intense cytoplasmic and perinuclear staining, coupled with enhanced nuclear localization, was observed in vSMCs, but not HepG2 cells, treated with benzo(a)pyrene (BaP), H or N-acetylcysteine, agents known to modulate redox status. 3′ RACE indicated that p70 is not generated as a product of endogenous gene expression, but rather taken up from the extracellular compartment. While p70 was undetectable in cells grown for 24 hours under serum-free conditions, cell-associated, acid-resistant albumin was detected 30 min after the addition of exogenous albumin. vSMCs incubated at 4°C with 100 g/mL unlabeled BSA and 10 g/mL FITC-BSA for 60 minutes and switched to 37°C to examine temperature-sensitive label uptake showed punctate structures throughout the cell consistent with albumin internalization at the higher temperature. Albumin was found to influence redox-signaling, as evidenced by modulation of cyp1a1 gsta1 and Ha-ras gene inducibility. Together, these results implicate albumin and albumin-like proteins as critical regulators of vascular redox signaling.

AB - This laboratory previously identified an albumin-like protein (denoted as p70) as a component of the macromolecular complex assembled within the 5′-regulatory region of redox-sensitive genes in vascular smooth muscle cells (vSMCs). Here we show that p70 is present in the cytosolic and nuclear compartments of vSMCs and dynamically responsive to redox status. Intense cytoplasmic and perinuclear staining, coupled with enhanced nuclear localization, was observed in vSMCs, but not HepG2 cells, treated with benzo(a)pyrene (BaP), H or N-acetylcysteine, agents known to modulate redox status. 3′ RACE indicated that p70 is not generated as a product of endogenous gene expression, but rather taken up from the extracellular compartment. While p70 was undetectable in cells grown for 24 hours under serum-free conditions, cell-associated, acid-resistant albumin was detected 30 min after the addition of exogenous albumin. vSMCs incubated at 4°C with 100 g/mL unlabeled BSA and 10 g/mL FITC-BSA for 60 minutes and switched to 37°C to examine temperature-sensitive label uptake showed punctate structures throughout the cell consistent with albumin internalization at the higher temperature. Albumin was found to influence redox-signaling, as evidenced by modulation of cyp1a1 gsta1 and Ha-ras gene inducibility. Together, these results implicate albumin and albumin-like proteins as critical regulators of vascular redox signaling.

UR - http://www.scopus.com/inward/record.url?scp=84874625269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874625269&partnerID=8YFLogxK

U2 - 10.1155/2013/628615

DO - 10.1155/2013/628615

M3 - Article

C2 - 23476722

AN - SCOPUS:84874625269

JO - Oxidative Medicine and Cellular Longevity

JF - Oxidative Medicine and Cellular Longevity

SN - 1942-0900

M1 - 628615

ER -