Algae as an electron donor promoting sulfate reduction for the bioremediation of acid rock drainage

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu2+. Sulfate, sulfide, Cu2+ and pH were monitored throughout the experiment of 123 d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80% of sulfate removal (12.7 mg SO4 2- d-1) enabling near complete Cu removal (>99.5%) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu2+ removal were observed in the endogenous control. In algae amended-columns, Cu2+ was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.

Original languageEnglish (US)
Pages (from-to)335-343
Number of pages9
JournalJournal of Hazardous Materials
Volume317
DOIs
StatePublished - Nov 5 2016

Fingerprint

Environmental Biodegradation
Bioremediation
Algae
bioremediation
Sulfates
Drainage
alga
Rocks
Electrons
drainage
sulfate
electron
Acids
acid
rock
Biofuels
Lipids
lipid
Alkalinity
Biodiesel

Keywords

  • Acid mine drainage
  • Algae waste
  • Biodiesel
  • Heavy metal
  • Permeable reactive barrier

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

Cite this

@article{d21d686ba810468cafdfa38ba633fee2,
title = "Algae as an electron donor promoting sulfate reduction for the bioremediation of acid rock drainage",
abstract = "This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu2+. Sulfate, sulfide, Cu2+ and pH were monitored throughout the experiment of 123 d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80{\%} of sulfate removal (12.7 mg SO4 2- d-1) enabling near complete Cu removal (>99.5{\%}) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu2+ removal were observed in the endogenous control. In algae amended-columns, Cu2+ was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.",
keywords = "Acid mine drainage, Algae waste, Biodiesel, Heavy metal, Permeable reactive barrier",
author = "Pedro Ayala-Parra and {Sierra Alvarez}, {Maria Reye} and Field, {James A}",
year = "2016",
month = "11",
day = "5",
doi = "10.1016/j.jhazmat.2016.06.011",
language = "English (US)",
volume = "317",
pages = "335--343",
journal = "Journal of Hazardous Materials",
issn = "0304-3894",
publisher = "Elsevier",

}

TY - JOUR

T1 - Algae as an electron donor promoting sulfate reduction for the bioremediation of acid rock drainage

AU - Ayala-Parra, Pedro

AU - Sierra Alvarez, Maria Reye

AU - Field, James A

PY - 2016/11/5

Y1 - 2016/11/5

N2 - This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu2+. Sulfate, sulfide, Cu2+ and pH were monitored throughout the experiment of 123 d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80% of sulfate removal (12.7 mg SO4 2- d-1) enabling near complete Cu removal (>99.5%) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu2+ removal were observed in the endogenous control. In algae amended-columns, Cu2+ was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.

AB - This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu2+. Sulfate, sulfide, Cu2+ and pH were monitored throughout the experiment of 123 d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80% of sulfate removal (12.7 mg SO4 2- d-1) enabling near complete Cu removal (>99.5%) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu2+ removal were observed in the endogenous control. In algae amended-columns, Cu2+ was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.

KW - Acid mine drainage

KW - Algae waste

KW - Biodiesel

KW - Heavy metal

KW - Permeable reactive barrier

UR - http://www.scopus.com/inward/record.url?scp=84974827266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84974827266&partnerID=8YFLogxK

U2 - 10.1016/j.jhazmat.2016.06.011

DO - 10.1016/j.jhazmat.2016.06.011

M3 - Article

C2 - 27318730

AN - SCOPUS:84974827266

VL - 317

SP - 335

EP - 343

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

ER -