Abstract
This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu2+. Sulfate, sulfide, Cu2+ and pH were monitored throughout the experiment of 123 d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80% of sulfate removal (12.7 mg SO4 2- d-1) enabling near complete Cu removal (>99.5%) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu2+ removal were observed in the endogenous control. In algae amended-columns, Cu2+ was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 335-343 |
| Number of pages | 9 |
| Journal | Journal of Hazardous Materials |
| Volume | 317 |
| DOIs | |
| State | Published - Nov 5 2016 |
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Keywords
- Acid mine drainage
- Algae waste
- Biodiesel
- Heavy metal
- Permeable reactive barrier
ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis
- Pollution
- Waste Management and Disposal
- Environmental Chemistry
- Environmental Engineering
Cite this
Algae as an electron donor promoting sulfate reduction for the bioremediation of acid rock drainage. / Ayala-Parra, Pedro; Sierra Alvarez, Maria Reye; Field, James A.
In: Journal of Hazardous Materials, Vol. 317, 05.11.2016, p. 335-343.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Algae as an electron donor promoting sulfate reduction for the bioremediation of acid rock drainage
AU - Ayala-Parra, Pedro
AU - Sierra Alvarez, Maria Reye
AU - Field, James A
PY - 2016/11/5
Y1 - 2016/11/5
N2 - This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu2+. Sulfate, sulfide, Cu2+ and pH were monitored throughout the experiment of 123 d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80% of sulfate removal (12.7 mg SO4 2- d-1) enabling near complete Cu removal (>99.5%) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu2+ removal were observed in the endogenous control. In algae amended-columns, Cu2+ was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.
AB - This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu2+. Sulfate, sulfide, Cu2+ and pH were monitored throughout the experiment of 123 d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80% of sulfate removal (12.7 mg SO4 2- d-1) enabling near complete Cu removal (>99.5%) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu2+ removal were observed in the endogenous control. In algae amended-columns, Cu2+ was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.
KW - Acid mine drainage
KW - Algae waste
KW - Biodiesel
KW - Heavy metal
KW - Permeable reactive barrier
UR - http://www.scopus.com/inward/record.url?scp=84974827266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84974827266&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2016.06.011
DO - 10.1016/j.jhazmat.2016.06.011
M3 - Article
C2 - 27318730
AN - SCOPUS:84974827266
VL - 317
SP - 335
EP - 343
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
ER -