Alkaline phosphatase 2 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea

J. Wei, M. Zhang, G. Liang, Xianchun Li

Research output: Contribution to journalArticle

Abstract

Although membrane-bound alkaline phosphatases (ALPs) have been proposed as a receptor for Cry1Ac in a few lepidopteran species, their potential functions as a Cry2Ab receptor are yet to be verified. To determine if ALP2 also serves as a receptor for Cry1Ac and even for Cry2Ab in Helicoverpa zea, we measured the potency of activated Cry1Ac and Cry2Ab against midgut and fat body cell lines of H. zea and the ovarian cell line of Spodoptera frugiperda (Sf9) expressing H. zea ALP2 (HzALP2) or transfected with HzALP2 double-stranded RNA (dsRNA). Relative to the control cells, the three cell lines expressing HzALP2 were more susceptible to Cry1Ac but there was no difference for Cry2Ab. By contrast, the two H. zea cell lines transfected with HzALP2 dsRNA were resistant to Cry1Ac while kept susceptible to Cry2Ab. Furthermore, RNA interference knockdown of HzALP2 in H. zea larvae enhanced larval survival on Cry1Ac-containing diets. These findings indicate that HzALP2 functions as a receptor of Cry1Ac but not Cry2Ab.

Original languageEnglish (US)
JournalInsect Molecular Biology
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Zea
Helicoverpa zea
Zea mays
Alkaline Phosphatase
alkaline phosphatase
receptors
cell lines
Cell Line
double-stranded RNA
Double-Stranded RNA
Spodoptera frugiperda
Spodoptera
Fat Body
fat body
RNA interference
midgut
RNA Interference
Adipocytes
Larva
Lepidoptera

Keywords

  • Bt-transgenic crops
  • cell toxicity
  • cross-resistance
  • gain-of-function analysis
  • pyramid
  • RNAi

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

Alkaline phosphatase 2 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea. / Wei, J.; Zhang, M.; Liang, G.; Li, Xianchun.

In: Insect Molecular Biology, 01.01.2019.

Research output: Contribution to journalArticle

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