Alloxan-glucose interaction: Effect on incorporation of14C-leucine into pancreatic islets of rat

Renu Bansal, Nafees Ahmad, Jalil R. Kidwai

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The acute effect of alloxan on the incorporation of14C-leucine into isolated rat islets of Langerhans was studied. I.v. administration of alloxan (40 mg/kg body weight) in rats inhibited the subsequent in vitro incorporation of14C-leucine into (pro-)insulin in the isolated islets. Glucose (750 mg/kg body weight), when administered 5 min prior to alloxan, completely protected the islets against alloxan toxicity. The protective effect of glucose was partly reversed when the concentration of alloxan was raised to 80 mg/kg body weight. Similar results of inhibition of (pro-)insulin biosynthesis by alloxan and its protection by glucose were obtained when isolated rat islets were exposed to alloxan and/or glucose in vitro. Islets exposed to glucose in vitro immediately after alloxan exposure showed a slower rate of inhibition of (pro-)insulin biosynthesis, as compared to islets washed before exposure to D-glucose. In view of these findings, it is suggested that there is a common recognition site on B-cell for alloxan and glucose.

Original languageEnglish (US)
Pages (from-to)135-143
Number of pages9
JournalActa Diabetologica Latina
Volume17
Issue number2
DOIs
StatePublished - Apr 1 1980
Externally publishedYes

Keywords

  • (Pro-) insulin
  • Alloxan
  • D-glucose
  • Islets of Langerhans
  • Pancreas

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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