Alpha-globulins suppress human leukocyte tumor necrosis factor secretion

P. Scuderi, Robert T Dorr, J. D. Liddil, P. R. Finley, P. Meltzer, A. B. Raitano, J. Rybski

Research output: Contribution to journalArticle

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Abstract

The secretion of tumor necrosis factor (TNF) by human peripheral blood mononuclear cells was suppressed by either whole human plasma α-globulins or purified α1-acid-glycoprotein, α1-antitrypsin and α2-macroglobulin in a concentration-dependent manner. α1-Antitrypsin was found to be the most suppressive of the purified proteins tested and completely blocked TNF release at concentrations above 1.25 mg/ml. Both α1-acid glycoprotein and α1-antitrypsin blocked TNF secretion by leukocytes which were simultaneously stimulated with either recombinant human interferon-γ (IFN-γ) or lipopolysaccharide (LPS). IFN-γ and LPS-activated cells were also susceptible to suppression mediated by these two α-globulins and the inhibition produced by 5 mg/ml α1-antitrypsin was greater than that caused by either 1 μM prostaglandin E2 or 10 ng/ml transforming growth factor-β1. The level of TNF mRNA in TNF-secreting and α-globulin-suppressed cells was examined and found to be to equal in both groups. The suppressive effect of whole α-globulins was confined to the inhibition of TNF secretion and these plasma proteins had no effect on the cytolytic activity of the recombinant cytokine as measured on murine L-929 target cells. Thus the α-globulins, which are a major fraction of the circulating plasma proteins, may function in TNF homeostasis by controlling TNF secretion without inhibiting the biological activity of the released cytokine.

Original languageEnglish (US)
Pages (from-to)939-942
Number of pages4
JournalEuropean Journal of Immunology
Volume19
Issue number5
StatePublished - 1989

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Alpha-Globulins
Leukocytes
Globulins
Tumor Necrosis Factor-alpha
Interferons
Lipopolysaccharides
Glycoproteins
Cytokines
Macroglobulins
Acids
Transforming Growth Factors
human TNF protein
Dinoprostone
Blood Proteins
Blood Cells
Homeostasis
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Scuderi, P., Dorr, R. T., Liddil, J. D., Finley, P. R., Meltzer, P., Raitano, A. B., & Rybski, J. (1989). Alpha-globulins suppress human leukocyte tumor necrosis factor secretion. European Journal of Immunology, 19(5), 939-942.

Alpha-globulins suppress human leukocyte tumor necrosis factor secretion. / Scuderi, P.; Dorr, Robert T; Liddil, J. D.; Finley, P. R.; Meltzer, P.; Raitano, A. B.; Rybski, J.

In: European Journal of Immunology, Vol. 19, No. 5, 1989, p. 939-942.

Research output: Contribution to journalArticle

Scuderi, P, Dorr, RT, Liddil, JD, Finley, PR, Meltzer, P, Raitano, AB & Rybski, J 1989, 'Alpha-globulins suppress human leukocyte tumor necrosis factor secretion', European Journal of Immunology, vol. 19, no. 5, pp. 939-942.
Scuderi P, Dorr RT, Liddil JD, Finley PR, Meltzer P, Raitano AB et al. Alpha-globulins suppress human leukocyte tumor necrosis factor secretion. European Journal of Immunology. 1989;19(5):939-942.
Scuderi, P. ; Dorr, Robert T ; Liddil, J. D. ; Finley, P. R. ; Meltzer, P. ; Raitano, A. B. ; Rybski, J. / Alpha-globulins suppress human leukocyte tumor necrosis factor secretion. In: European Journal of Immunology. 1989 ; Vol. 19, No. 5. pp. 939-942.
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