Alt a 1 allergen homologs from Alternaria and related taxa: Analysis of phylogenetic content and secondary structure

Soon Gyu Hong, Robert A. Cramer, Christopher B. Lawrence, Barry M Pryor

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

A gene for the Alternaria major allergen, Alt a 1, was amplified from 52 species of Alternaria and related genera, and sequence information was used for phylogenetic study. Alt a 1 gene sequences evolved 3.8 times faster and contained 3.5 times more parsimony-informative sites than glyceraldehyde-3- phosphate dehydrogenase (gpd) sequences. Analyses of Alt a 1 gene and gpd exon sequences strongly supported grouping of Alternaria spp. and related taxa into several species-groups described in previous studies, especially the infectoria, alternata, porri, brassicicola, and radicina species-groups and the Embellisia group. The sonchi species-group was newly suggested in this study. Monophyly of the Nimbya group was moderately supported, and monophyly of the Ulocladium group was weakly supported. Relationships among species-groups and among closely related species of the same species-group were not fully resolved. However, higher resolution could be obtained using Alt a 1 sequences or a combined dataset than using gpd sequences alone. Despite high levels of variation in amino acid sequences, results of in silico prediction of protein secondary structure for Alt a 1 demonstrated a high degree of structural similarity for most of the species suggesting a conservation of function.

Original languageEnglish (US)
Pages (from-to)119-129
Number of pages11
JournalFungal Genetics and Biology
Volume42
Issue number2
DOIs
StatePublished - Feb 2005

Fingerprint

Alternaria
Glyceraldehyde-3-Phosphate Dehydrogenases
Allergens
Genes
Secondary Protein Structure
Computer Simulation
Amino Acid Sequence
Exons

Keywords

  • Alt a 1 allergen
  • Alternaria
  • gpd
  • Phylogenetics
  • Secondary structute

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

Alt a 1 allergen homologs from Alternaria and related taxa : Analysis of phylogenetic content and secondary structure. / Hong, Soon Gyu; Cramer, Robert A.; Lawrence, Christopher B.; Pryor, Barry M.

In: Fungal Genetics and Biology, Vol. 42, No. 2, 02.2005, p. 119-129.

Research output: Contribution to journalArticle

Hong, Soon Gyu ; Cramer, Robert A. ; Lawrence, Christopher B. ; Pryor, Barry M. / Alt a 1 allergen homologs from Alternaria and related taxa : Analysis of phylogenetic content and secondary structure. In: Fungal Genetics and Biology. 2005 ; Vol. 42, No. 2. pp. 119-129.
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