Alteration of Lens electrolyte transport parameters following transient oxidative perturbation

Nicholas A Delamere, C. A. Paterson, D. B. Borchman, S. K. Hensley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Rabbit lenses were incubated, in vitro, for 20 hr. During the first 60 min of the incubation period, the lenses were exposed to hydrogen peroxide at specified concentrations. Deranged electrolyte balance was observed in lenses exposed to hydrogen peroxide concentrations exceeding 0.1 mM. Lenticular sodium pump activity, as evidenced by lens 86Rb uptake, was reduced to 61% of control when examined immediately after exposure to 0.5 mM hydrogen peroxide for 1 hr. Upon further incubation in peroxide-free medium, the sodium pump activity of hydrogen peroxide treated lenses continued to deteriorate. The deterioration of sodium pump activity in lenses transiently challenged with hydrogen peroxide was not altered by supplementation of the incubation medium with antioxidants or by substitution of calcium in the medium. Lenticular potassium permeability, as evidenced by the rate of 86Rb efflux from the lens, was found to be elevated immediately after exposure to 0.5 mM hydrogen peroxide for 1 hr. However, no further change in the rate of 86Rb efflux was noted when the hydrogen peroxide treated lens was incubated for an additional 19 hr in peroxide-free medium. Na, K-ATPase and Ca-ATPase activities were measured in membrane preparations isolated from lenses that had been exposed to 0.5 mM hydrogen peroxide and then cultured in control medium for 19 hr. Neither of the transport enzyme activities was diminished in the hydrogen peroxide treated lenses indicating that the deterioration in sodium pump activity in lenses temporarily exposed to hydrogen peroxide is not due to a progressive loss of Na, K-ATPase enzyme.

Original languageEnglish (US)
Pages (from-to)969-979
Number of pages11
JournalCurrent Eye Research
Volume7
Issue number10
DOIs
StatePublished - 1988
Externally publishedYes

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Electrolytes
Lenses
Hydrogen Peroxide
Sodium-Potassium-Exchanging ATPase
Peroxides
Water-Electrolyte Balance
Enzymes
Adenosine Triphosphatases
Permeability
Potassium
Antioxidants
Rabbits
Calcium
Membranes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Alteration of Lens electrolyte transport parameters following transient oxidative perturbation. / Delamere, Nicholas A; Paterson, C. A.; Borchman, D. B.; Hensley, S. K.

In: Current Eye Research, Vol. 7, No. 10, 1988, p. 969-979.

Research output: Contribution to journalArticle

Delamere, Nicholas A ; Paterson, C. A. ; Borchman, D. B. ; Hensley, S. K. / Alteration of Lens electrolyte transport parameters following transient oxidative perturbation. In: Current Eye Research. 1988 ; Vol. 7, No. 10. pp. 969-979.
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