Alteration of tropomyosin-binding properties of tropomodulin-1 affects its capping ability and localization in skeletal myocytes

Natalia A. Moroz, Stefanie M. Novak, Ricardo Azevedo, Mert Colpan, Vladimir N. Uversky, Carol C. Gregorio, Alla S. Kostyukova

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Background: Tropomodulin is a tropomyosin-dependent actin-capping protein. Results: Mutations intropomodulin-1 that reduce its affinity for tropomyosin (R11K, D12N, Q144K) reduced inhibition of actin pointed-end polymerization in vitro and decreased assembly of tropomodulin-1 in skeletal myocytes. Conclusion: The tropomyosin-binding ability of tropomodulin-1 directly influences its actin filament regulatory activity. Significance: Creating a tool for studying the roles of different tropomodulin isoforms in living cells.

Original languageEnglish (US)
Pages (from-to)4899-4907
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number7
DOIs
StatePublished - Feb 15 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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