Alterations in kappa opioid receptor mRNA levels in the paraventricular nucleus of the hypothalamus by stress and sex steroids

Rustam Y. Yukhananov, Robert J. Handa

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In this study, the levels of kappa opioid receptor (kOR) mRNA were determined using in situ hybridization following two types of stress (i.p. injection of hypertonic saline or novelty). In addition, we examined the possibility that estrogen or androgen would modify kOR mRNA. Gonadectomized male rats treated with estrogen or dihydrotestosterone were sacrificed 45 min after spending 15 min in a novel open field, or 60 min following hypertonic saline injection. Two-way ANOVA revealed that estrogen and novelty increased the levels of kOR mRNA in the ventral zone of the medial parvocellular part of the paraventricular nucleus (PVN), but not in the lateral parvocellular part of the PVN, claustrum, nucleus accumbens or the nucleus of the lateral olfactory tract. Furthermore, novelty increased kOR mRNA in gonadectomized (GDX) and GDX rats treated with dihydrotestosterone (DHTP), but not in sham-operated or estrogen-treated animals. Taken together, these data indicate that kOR mRNA levels are under estrogenic control and up-regulated in a stressor specific fashion.

Original languageEnglish (US)
Pages (from-to)1690-1694
Number of pages5
JournalNeuroReport
Volume7
Issue number10
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Keywords

  • Androgen
  • Estrogen
  • Gonadal steroids
  • Kappa opioid receptor mRNA
  • Novelty
  • PVN
  • Stress: nucleus accumbens

ASJC Scopus subject areas

  • Neuroscience(all)

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