Altered growth and root tip morphology in Pisum sativum L. in response to altered expression of a gene expressed in border cells

Fushi Wen, Lindy A. Brigham, Gilberto Curlango-Rivera, Zhongguo Xiong, Martha C Hawes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background and aims: Recent studies suggest that root border cells function in defense of the root by an extracellular DNA-based trapping mechanism similar to that described in mammalian white blood cells. Genes controlling the specialized properties of border cells as they detach from the root tip therefore are of interest. Methods: mRNA differential display was used to identify Brd13, a sequence expressed in border cells but not other root tissues. RNase protection and mRNA Northern blot analyses, and reporter gene expression under the control of the Brd13 promoter in transgenic hairy roots were used to confirm localized expression. Phenotype analysis of transgenic hairy roots expressing Brd13 antisense mRNA was carried out. Results: Brd13 was expressed constitutively in border cells but not in leaves, stems, or roots without border cells. The predicted protein shares sequence similarity with flavin-binding proteins. Transgenic hairy roots expressing Brd13 antisense mRNA exhibited abnormal growth and morphology. Conclusions: We report here that altered expression of a putative flavin-binding protein in border cells resulted in altered root development. Flavin-binding proteins play key roles in development, defense, and local auxin biosynthesis. The Brd13 gene and its promoter may be useful in creating defined changes in root development and defense.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalPlant and Soil
Volume377
Issue number1-2
DOIs
StatePublished - 2014

Fingerprint

root tips
Pisum sativum
gene expression
gene
flavins
cells
binding proteins
protein
genetically modified organisms
promoter regions
border
reporter genes
Northern blotting
trapping
auxins
leukocytes
genes
phenotype
biosynthesis
blood

Keywords

  • Extracellular trapping
  • Flavin-binding protein
  • Pisum sativum L
  • Root border cells
  • Root cap

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

Cite this

Altered growth and root tip morphology in Pisum sativum L. in response to altered expression of a gene expressed in border cells. / Wen, Fushi; Brigham, Lindy A.; Curlango-Rivera, Gilberto; Xiong, Zhongguo; Hawes, Martha C.

In: Plant and Soil, Vol. 377, No. 1-2, 2014, p. 179-187.

Research output: Contribution to journalArticle

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AU - Xiong, Zhongguo

AU - Hawes, Martha C

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