Amino acid levels across normal forearm muscle and splanchnic bed after a protein meal

T. T. Aoki, M. F. Brennan, W. A. Müller, J. S. Soeldner, J. S. Alpert, S. B. Saltz, R. L. Kaufmann, M. H. Tan, G. F. Cahill

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Abstract

To characterize further the role of blood cells in amino acid transport, four normal volunteers were asked to ingest 200 g of broiled ground sirloin within a 10 min period. Blood samples from a radial artery, a deep vein draining forearm muscle bed, and a tributary of the hepatic vein were obtained prior to and for 4 hr after the meal and analyzed for various hormones and substrates. At rest, analysis of arterio deep venous differences across the forearm revealed the blood cell and plasma amino acid compartmental contents to be relatively constant except for alanine and glutamine. Whole blood arterio hepatic venous (A HV) amino acid differences were not significantly different from zero; however, blood cell A HV difference analysis revealed a significant release of threonine from the splanchnic bed while plasma A HV difference analysis revealed modest but significant releases of glutamate and ornithine and uptakes of phenylalanine and histidine. After the ingestion of the meat meal, plasma and blood cell levels of almost all of the measured amino acids increased significantly. Notable exceptions included glycine and alanine, levels of which did not change significantly, and glutamine. The arterial blood cell content of the latter amino acid paradoxically decreased while arterial plasma levels increased significantly. Most importantly, large quantities of branched chain amino acids were released from the splanchic bed and removed by forearm muscle. These data suggest that the blood cells of normal man do actively participate in amino acid transport, and that the magnitude and direction of change induced by the ingestion of a protein meal varies with the individual amino acid.

Original languageEnglish (US)
Pages (from-to)340-350
Number of pages11
JournalUnknown Journal
Volume29
Issue number4
DOIs
StatePublished - Jan 1 1976

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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    Aoki, T. T., Brennan, M. F., Müller, W. A., Soeldner, J. S., Alpert, J. S., Saltz, S. B., Kaufmann, R. L., Tan, M. H., & Cahill, G. F. (1976). Amino acid levels across normal forearm muscle and splanchnic bed after a protein meal. Unknown Journal, 29(4), 340-350. https://doi.org/10.1093/ajcn/29.4.340