Amperometric measurements of catecholamine release from single vesicles in MN9D cells

Yan Dong, Michael L Heien, Marc M. Maxson, Andrew G. Ewing

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

MN9D cells have been used as a successful model to investigate dopamine pharmacology and to test the specific effects of drugs for the treatment of Parkinson's disease. However, quantitative measurements of quantal release from these cells have not been carried out. In this work, we used amperometry to investigate catecholamine release from MN9D cells. Amperometric events were observed in both undifferentiated and differentiated (butyric acid-treated) cells. An increase in quantal size and half-width was observed for differentiated cells versus undifferentiated cells; however, the number of events per cell and the amplitude remained constant. In transmission electron microscopy images, no obvious cluster of small synaptic vesicles was observed, and large dense-core vesicles were present in the cell body of undifferentiated cells; however, after differentiation, vesicles were concentrated in the cell processes. In differentiated cells, l-DOPA caused an increase in quantal size and half-width, which could be blocked by the vesicular monoamine transporter inhibitor, reserpine.

Original languageEnglish (US)
Pages (from-to)1589-1595
Number of pages7
JournalJournal of Neurochemistry
Volume107
Issue number6
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

Vesicular Monoamine Transport Proteins
Butyric Acid
Reserpine
Catecholamines
Dopamine
Cells
Transmission electron microscopy
Pharmaceutical Preparations
Synaptic Vesicles
Secretory Vesicles
Transmission Electron Microscopy
Parkinson Disease

Keywords

  • Catecholamine
  • Dopamine
  • Half-width
  • MD9D cells
  • Parkinson's disease
  • Quantal size

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Amperometric measurements of catecholamine release from single vesicles in MN9D cells. / Dong, Yan; Heien, Michael L; Maxson, Marc M.; Ewing, Andrew G.

In: Journal of Neurochemistry, Vol. 107, No. 6, 12.2008, p. 1589-1595.

Research output: Contribution to journalArticle

Dong, Yan ; Heien, Michael L ; Maxson, Marc M. ; Ewing, Andrew G. / Amperometric measurements of catecholamine release from single vesicles in MN9D cells. In: Journal of Neurochemistry. 2008 ; Vol. 107, No. 6. pp. 1589-1595.
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