Amplifying small amounts of tumor DNA allows detection of DNA copy number aberrations with array-CGH

Lars Prestegarden, Anjan Misra, Marcus L. Ware, Ru Fang Yeh, Rolf Bjerkvig, Burt G. Feuerstein

Research output: Contribution to journalArticle

Abstract

Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.

Original languageEnglish (US)
Pages (from-to)3-6
Number of pages4
JournalBioTechniques
Volume44
Issue number7
DOIs
StatePublished - Jun 2008

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

Fingerprint Dive into the research topics of 'Amplifying small amounts of tumor DNA allows detection of DNA copy number aberrations with array-CGH'. Together they form a unique fingerprint.

  • Cite this