Amyloid-like β-aggregates as force-sensitive switches in fungal biofilms and infections

Peter N. Lipke, Stephen A Klotz, Yves F. Dufrene, Desmond N. Jackson, Melissa C. Garcia-Sherman

Research output: Contribution to journalReview article

15 Citations (Scopus)

Abstract

Cellular aggregation is an essential step in the formation of biofilms, which promote fungal survival and persistence in hosts. In many of the known yeast cell adhesion proteins, there are amino acid sequences predicted to form amyloidlike-aggregates. These sequences mediate amyloid formation in vitro. In vivo, these sequences mediate a phase transition from a disordered state to a partially ordered state to create patches of adhesins on the cell surface. These-aggregated protein patches are called adhesin nanodomains, and their presence greatly increases and strengthens cell-cell interactions in fungal cell aggregation. Nanodomain formation is slow (with molecular response in minutes and the consequences being evident for hours), and strong interactions lead to enhanced biofilm formation. Unique among functional amyloids, fungal adhesin-aggregation can be triggered by the application of physical shear force, leading to cellular responses to flow-induced stress and the formation of robust biofilms that persist under flow. Bioinformatics analysis suggests that this phenomenon may be widespread. Analysis of fungal abscesses shows the presence of surface amyloids in situ, a finding which supports the idea that phase changes to an amyloid-like state occur in vivo. The amyloid-coated fungi bind the damage-associated molecular pattern receptor serum amyloid P component, and there may be a consequential modulation of innate immune responses to the fungi. Structural data now suggest mechanisms for the force-mediated induction of the phase change. We summarize and discuss evidence that the sequences function as triggers for protein aggregation and subsequent cellular aggregation, both in vitro and in vivo.

Original languageEnglish (US)
Article numbere00035-17
JournalMicrobiology and Molecular Biology Reviews
Volume82
Issue number1
DOIs
StatePublished - Mar 1 2018

Fingerprint

Mycoses
Biofilms
Amyloid
Fungi
Serum Amyloid P-Component
Cell Aggregation
Proteins
Phase Transition
Computational Biology
Innate Immunity
Cell Adhesion
Cell Communication
Abscess
Amino Acid Sequence
Yeasts
In Vitro Techniques

Keywords

  • Adhesins
  • Amyloids
  • Avidity
  • Beta aggregation
  • Candida albicans
  • Catch bonding
  • Mannoproteins
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology and Microbiology(all)

Cite this

Amyloid-like β-aggregates as force-sensitive switches in fungal biofilms and infections. / Lipke, Peter N.; Klotz, Stephen A; Dufrene, Yves F.; Jackson, Desmond N.; Garcia-Sherman, Melissa C.

In: Microbiology and Molecular Biology Reviews, Vol. 82, No. 1, e00035-17, 01.03.2018.

Research output: Contribution to journalReview article

Lipke, Peter N. ; Klotz, Stephen A ; Dufrene, Yves F. ; Jackson, Desmond N. ; Garcia-Sherman, Melissa C. / Amyloid-like β-aggregates as force-sensitive switches in fungal biofilms and infections. In: Microbiology and Molecular Biology Reviews. 2018 ; Vol. 82, No. 1.
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