An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

Mengxing Ouyang, Ruchika Mohan, Yi Lu, Tingting Liu, Kathleen E. Mach, Mandy L Y Sin, Mason McComb, Janhvi Joshi, Vincent Gau, Pak Kin Wong, Joseph C. Liao

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cassette to enhance molecular convection and hybridization efficiency through electrokinetics induced fluid motion and Joule heating induced temperature elevation. Using E. coli as an exemplary pathogen, we determined the optimal electrokinetic parameters for detecting bacterial 16S rRNA in the biosensor cassette based on the current output, signal-to-noise ratio, and limit of detection. In addition, a panel of six probe sets targeting common uropathogenic bacteria was demonstrated. The optimized parameters were also validated using patient-derived clinical urine samples. The effectiveness of electrokinetics for on chip sample preparation will facilitate the implementation of point-of-care diagnosis of urinary tract infection in the future.

Original languageEnglish (US)
Pages (from-to)3660-3666
Number of pages7
JournalAnalyst
Volume138
Issue number13
DOIs
StatePublished - Jul 7 2013

Fingerprint

Biosensing Techniques
Pathogens
Point-of-Care Systems
Biosensors
pathogen
Urinary Tract Infections
sample preparation
Convection
Joule heating
Fluidics
Signal-To-Noise Ratio
Heating
Escherichia coli
Communicable Diseases
Limit of Detection
Signal to noise ratio
Bacteria
infectious disease
Urine
urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Ouyang, M., Mohan, R., Lu, Y., Liu, T., Mach, K. E., Sin, M. L. Y., ... Liao, J. C. (2013). An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification. Analyst, 138(13), 3660-3666. https://doi.org/10.1039/c3an00259d

An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification. / Ouyang, Mengxing; Mohan, Ruchika; Lu, Yi; Liu, Tingting; Mach, Kathleen E.; Sin, Mandy L Y; McComb, Mason; Joshi, Janhvi; Gau, Vincent; Wong, Pak Kin; Liao, Joseph C.

In: Analyst, Vol. 138, No. 13, 07.07.2013, p. 3660-3666.

Research output: Contribution to journalArticle

Ouyang, M, Mohan, R, Lu, Y, Liu, T, Mach, KE, Sin, MLY, McComb, M, Joshi, J, Gau, V, Wong, PK & Liao, JC 2013, 'An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification', Analyst, vol. 138, no. 13, pp. 3660-3666. https://doi.org/10.1039/c3an00259d
Ouyang M, Mohan R, Lu Y, Liu T, Mach KE, Sin MLY et al. An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification. Analyst. 2013 Jul 7;138(13):3660-3666. https://doi.org/10.1039/c3an00259d
Ouyang, Mengxing ; Mohan, Ruchika ; Lu, Yi ; Liu, Tingting ; Mach, Kathleen E. ; Sin, Mandy L Y ; McComb, Mason ; Joshi, Janhvi ; Gau, Vincent ; Wong, Pak Kin ; Liao, Joseph C. / An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification. In: Analyst. 2013 ; Vol. 138, No. 13. pp. 3660-3666.
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