An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

Mengxing Ouyang, Ruchika Mohan, Yi Lu, Tingting Liu, Kathleen E. Mach, Mandy L.Y. Sin, Mason McComb, Janhvi Joshi, Vincent Gau, Pak Kin Wong, Joseph C. Liao

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cassette to enhance molecular convection and hybridization efficiency through electrokinetics induced fluid motion and Joule heating induced temperature elevation. Using E. coli as an exemplary pathogen, we determined the optimal electrokinetic parameters for detecting bacterial 16S rRNA in the biosensor cassette based on the current output, signal-to-noise ratio, and limit of detection. In addition, a panel of six probe sets targeting common uropathogenic bacteria was demonstrated. The optimized parameters were also validated using patient-derived clinical urine samples. The effectiveness of electrokinetics for on chip sample preparation will facilitate the implementation of point-of-care diagnosis of urinary tract infection in the future.

Original languageEnglish (US)
Pages (from-to)3660-3666
Number of pages7
JournalAnalyst
Volume138
Issue number13
DOIs
StatePublished - Jul 7 2013

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of 'An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification'. Together they form a unique fingerprint.

  • Cite this

    Ouyang, M., Mohan, R., Lu, Y., Liu, T., Mach, K. E., Sin, M. L. Y., McComb, M., Joshi, J., Gau, V., Wong, P. K., & Liao, J. C. (2013). An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification. Analyst, 138(13), 3660-3666. https://doi.org/10.1039/c3an00259d