An Assay Suitable for High Throughput Screening of Anti-Influenza Drugs

Lili Mao, Jun Wang, William F. DeGrado, Masayori Inouye

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.

Original languageEnglish (US)
Article numbere54070
JournalPloS one
Volume8
Issue number1
DOIs
StatePublished - Jan 17 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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