An Electrochemical Biosensor for Rapid Detection of Pediatric Bloodstream Infections

Eranda M.K. Kurundu Hewage, Debbie Spear, Todd M. Umstead, Sanmei Hu, Ming Wang, Pak Kin Wong, Zissis C. Chroneos, E. Scott Halstead, Neal J. Thomas

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Bloodstream infections are major contributing factors of morbidity and mortality among children. Precise and timely identification of causative agents can improve the clinical management and outcome of the infection, potentially saving lives. Electrochemical biosensors previously described by Gao et al. (2017) have the potential to deliver greater speed and discrimination. However, to date there are no data that determine whether the age of the host would cause bacteria to demonstrate different growth characteristics, or whether pediatric samples would behave differently using this electrochemical biosensor. The importance of this knowledge gap is clear: the preclinical testing phase of this line of research is limited by the relative lack of pediatric healthy blood volunteers to complete this work. Therefore, in this study we have applied this novel technology to diagnose bacteria spiked into pediatric blood and compared directly with adult blood samples. Only 180 µL of blood was utilized from both adult and pediatric volunteers and inoculated with Escherichia coli 67, and the signals generated at different time points were compared. We were able to demonstrate that the signals generated by adult and pediatric blood were not significantly different with this detection technology.

Original languageEnglish (US)
Pages (from-to)616-625
Number of pages10
JournalSLAS Technology
Volume22
Issue number6
DOIs
StatePublished - Dec 1 2017

Keywords

  • bloodstream infections
  • electrochemical biosensor
  • pediatric blood
  • sepsis

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

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  • Cite this

    Kurundu Hewage, E. M. K., Spear, D., Umstead, T. M., Hu, S., Wang, M., Wong, P. K., Chroneos, Z. C., Halstead, E. S., & Thomas, N. J. (2017). An Electrochemical Biosensor for Rapid Detection of Pediatric Bloodstream Infections. SLAS Technology, 22(6), 616-625. https://doi.org/10.1177/2472630317727704