Abstract
This study reports a hybrid electrokinetic microdevice for rapid concentration and detection of circulating cell-free (cf)DNA. Rapid molecular analysis of cfDNA has the potential to change the current practice of medicine, such as in cancer diagnostics and in monitoring the efficacy of cancer treatments. With a combination of AC electrothermal flow and dielectrophoresis, the hybrid electrokinetic microdevice efficiently concentrates cfDNA from blood plasma and other physiological fluids. In this design, the long-range AC electrothermal flow, which is effective in conductive fluids, drives the cfDNA towards the center of the electrode where dielectrophoretic trapping of the cfDNA occurs. Once the cfDNA is collected at the electrode, the concentration in the blood sample can be quantified by fluorescence analysis with an intercalating dye that binds specifically to double-stranded DNA. The effects of the electrokinetic parameters were elucidated to optimize the design of the device. The device was demonstrated to separate high molecular weight DNA from low molecular weight DNA. Quantitative detection of clinically relevant concentrations of cfDNA was achieved in 10 minutes.
| Original language | English (US) |
|---|---|
| Title of host publication | 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 544-547 |
| Number of pages | 4 |
| ISBN (Print) | 9781479989553 |
| DOIs | |
| State | Published - Aug 5 2015 |
| Event | 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States Duration: Jun 21 2015 → Jun 25 2015 |
Other
| Other | 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 |
|---|---|
| Country | United States |
| City | Anchorage |
| Period | 6/21/15 → 6/25/15 |
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Keywords
- AC electrokinetics
- AC electrothermal flow
- cancer
- circulating cell-free DNA
- dielectrophoresis
ASJC Scopus subject areas
- Instrumentation
- Electrical and Electronic Engineering
Cite this
An electrokinetic microdevice for isolation and quantification of circulating cell-free DNA from physiological samples. / Lamanda, Ariana; Lu, Yi; Gill, Navrose; Wong, Pak Kin.
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 544-547 7180981.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - An electrokinetic microdevice for isolation and quantification of circulating cell-free DNA from physiological samples
AU - Lamanda, Ariana
AU - Lu, Yi
AU - Gill, Navrose
AU - Wong, Pak Kin
PY - 2015/8/5
Y1 - 2015/8/5
N2 - This study reports a hybrid electrokinetic microdevice for rapid concentration and detection of circulating cell-free (cf)DNA. Rapid molecular analysis of cfDNA has the potential to change the current practice of medicine, such as in cancer diagnostics and in monitoring the efficacy of cancer treatments. With a combination of AC electrothermal flow and dielectrophoresis, the hybrid electrokinetic microdevice efficiently concentrates cfDNA from blood plasma and other physiological fluids. In this design, the long-range AC electrothermal flow, which is effective in conductive fluids, drives the cfDNA towards the center of the electrode where dielectrophoretic trapping of the cfDNA occurs. Once the cfDNA is collected at the electrode, the concentration in the blood sample can be quantified by fluorescence analysis with an intercalating dye that binds specifically to double-stranded DNA. The effects of the electrokinetic parameters were elucidated to optimize the design of the device. The device was demonstrated to separate high molecular weight DNA from low molecular weight DNA. Quantitative detection of clinically relevant concentrations of cfDNA was achieved in 10 minutes.
AB - This study reports a hybrid electrokinetic microdevice for rapid concentration and detection of circulating cell-free (cf)DNA. Rapid molecular analysis of cfDNA has the potential to change the current practice of medicine, such as in cancer diagnostics and in monitoring the efficacy of cancer treatments. With a combination of AC electrothermal flow and dielectrophoresis, the hybrid electrokinetic microdevice efficiently concentrates cfDNA from blood plasma and other physiological fluids. In this design, the long-range AC electrothermal flow, which is effective in conductive fluids, drives the cfDNA towards the center of the electrode where dielectrophoretic trapping of the cfDNA occurs. Once the cfDNA is collected at the electrode, the concentration in the blood sample can be quantified by fluorescence analysis with an intercalating dye that binds specifically to double-stranded DNA. The effects of the electrokinetic parameters were elucidated to optimize the design of the device. The device was demonstrated to separate high molecular weight DNA from low molecular weight DNA. Quantitative detection of clinically relevant concentrations of cfDNA was achieved in 10 minutes.
KW - AC electrokinetics
KW - AC electrothermal flow
KW - cancer
KW - circulating cell-free DNA
KW - dielectrophoresis
UR - http://www.scopus.com/inward/record.url?scp=84955446832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955446832&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2015.7180981
DO - 10.1109/TRANSDUCERS.2015.7180981
M3 - Conference contribution
AN - SCOPUS:84955446832
SN - 9781479989553
SP - 544
EP - 547
BT - 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PB - Institute of Electrical and Electronics Engineers Inc.
ER -