An electrophoretic karyotype of Neurospora crassa.

M. J. Orbach, D. Vollrath, R. W. Davis, C. Yanofsky

Research output: Contribution to journalArticle

200 Scopus citations

Abstract

A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

Original languageEnglish (US)
Pages (from-to)1469-1473
Number of pages5
JournalMolecular and cellular biology
Volume8
Issue number4
DOIs
StatePublished - Apr 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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